Refer to the DNA Preparation Workflow for Ion Torrent tab below for a graphic illustration of the steps.
NEBNext® reagents are available for Ion Torrent DNA library preparation, with or without enzyme-based fragmentation. Each of these reagents has been functionally validated by preparation of a genomic DNA library followed by Ion Torrent sequencing. For representative results of both library construction using NEBNext and Ion Torrent run results, choose the tabs below.
Having trouble deciding which NEBNext Fast DNA Library Prep Set for Ion Torrent® to choose? Let Christine explain which set is right for your DNA.
Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab.
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.