OneTaq® DNA Polymerase
The ONE polymerase for your endpoint PCR needs. OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- 2X higher fidelity than Taq
- Ideal for routine, AT- or GC-rich templates
- Hot start and master mix versions available
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Product Information
The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1) . The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.
OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.
Figure 1: Comparison of OneTaq products to other commercially available polymerases
Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.
Figure 2: OneTaq Buffer recommendations
![[Icon] Use our Tm Calculator to determine your primers' optimal annealing temperature.](https://media.neb.com/asset/46e6c17d-deda-4972-a20c-2c3463d03d8b/Webp_JPG_PSD/TmCalculator_Banner.webp?=)
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.- This product is related to the following categories:
- OneTaq® DNA Polymerases,
- Taq DNA Polymerase
- This product can be used in the following applications:
- Multiplex PCR,
- Specialty PCR,
- Routine PCR, PCR
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Additional Citations
- Megan S Thoemmes, Daniel J Fergus, Julie Urban, Michelle Trautwein, Robert R Dunn (2014) Ubiquity and diversity of human-associated demodex mites. PLoS One; 9, e106265. PubMedID: 25162399, DOI: 10.1371/journal.pone.0106265
- Matousková, M., Vesely, P., Daniel, P., Mattiuzzo, G., Hector, R.D., Scobie, L., Takeuchi, Y. and Hejnar, J. (2013) Role of DNA methylation in expression and transmission of porcine endogenous retroviruses. PLoS One; PubMedID: 23986605
- Liang, Y., Basu, D., Pattathil, S., Xu, W.L., Venetos, A., Martin, S.L., Faik, A., Hahn, M.G. and Showalter, A.M. (2013) Biochemical and physiological characterization of fut4 and fut6 mutants defective in arabinogalactan-protein fucosylation in Arabidopsis. PLoS One; PubMedID: 24127514
- Malyshev, D.A., Dhami, K., Quach, H.T., Lavergne, T., Ordoukhanian, P., Torkamani, A. and Romesberg, F.E. (2012) Efficient and sequence-independent replication of DNA containing a third base pair establishes a functional six-letter genetic alphabet. Proc Natl Acad Sci U S A; 109, 4503-4508. PubMedID: 22773812
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