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UMass Medical School ASC

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Inventory as of 7/3/2020 2:42 AM

NEB # DNA Modifying Enzymes and Cloning Technologies Size Quantity Available
E2611S Gibson Assembly® Master Mix 10 reactions 2
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions 2
M2200S Quick Ligation™ Kit 30 reactions 3
M0202S T4 DNA Ligase 20,000 units 2
M0202L T4 DNA Ligase 100,000 units 2
NEB # Epigenetics Size Quantity Available
R0176S DpnI 1,000 units 2
R3138S SalI-HF® 2,000 units 2
NEB # Markers & Ladders Size Quantity Available
N3232S 1 kb DNA Ladder 0.2 ml 2
N3232L 1 kb DNA Ladder 1 ml 2
N3200S 1 kb Plus DNA Ladder 0.1 ml 3
N3231S 100 bp DNA Ladder 0.1 ml 2
N3231L 100 bp DNA Ladder 0.5 ml 2
N3233S Low Molecular Weight DNA Ladder 0.1 ml 1
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml 2
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml 2
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml 2
NEB # Nucleic Acid Purification Size Quantity Available
E1050L Exo-CIP™ Rapid PCR Cleanup Kit 400 reactions 1
E1050S Exo-CIP™ Rapid PCR Cleanup Kit 100 reactions 2
T1020S Monarch® DNA Gel Extraction Kit 50 preps 2
T1020L Monarch® DNA Gel Extraction Kit 250 preps 1
T3010S Monarch® Genomic DNA Purification Kit 50 preps 2
T3010L Monarch® Genomic DNA Purification Kit 150 preps 1
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps 1
T1030S Monarch® PCR & DNA Cleanup Kit (5 μg) 50 preps 2
T1010L Monarch® Plasmid Miniprep Kit  250 preps 1
T1010S Monarch® Plasmid Miniprep Kit  50 preps 2
NEB # PCR, Polymerases & Amplification Technologies Size Quantity Available
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units 2
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions 2
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) 2
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) 2
E3010S LunaScript™ RT SuperMix Kit 25 reactions 1
M0541S NEBNext® High-Fidelity 2X PCR Master Mix 50 reactions 2
M0530L Phusion® High-Fidelity DNA Polymerase 500 units 1
M0530S Phusion® High-Fidelity DNA Polymerase 100 units 2
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) 2
M0491L Q5® High-Fidelity DNA Polymerase 500 units 2
M0491S Q5® High-Fidelity DNA Polymerase 100 units 4
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units 2
M0203S T4 DNA Polymerase 150 units 2
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units 2
NEB # Protein Tools Size Quantity Available
P7719S Color Prestained Protein Standard, Broad Range (10-250 kDa) 150 gel lanes 1
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials 1
NEB # Restriction Endonucleases Size Quantity Available
R3552S AgeI-HF® 300 units 2
R0174S AvrII 100 units 2
R3136S BamHI-HF® 10,000 units 2
R0144S BglII 2,000 units 2
R0197S ClaI 1,000 units 2
R3505S EagI-HF® 500 units 1
R3101S EcoRI-HF® 10,000 units 2
R3195S EcoRV-HF® 4,000 units 2
R3104S HindIII-HF® 10,000 units 2
R3142S KpnI-HF® 4,000 units 2
R3131S NheI-HF® 1,000 units 2
R3189S NotI-HF® 500 units 3
R3189L NotI-HF® 2,500 units 1
R0547S PacI 250 units 2
R3150S PvuI-HF® 500 units 2
R3151S PvuII-HF® 5,000 units 2
R3156S SacI-HF® 2,000 units 2
R0157S SacII 2,000 units 2
R3122S ScaI-HF® 1,000 units 2
R0141S SmaI 2,000 units 2
R3133S SpeI-HF® 500 units 1
R3182S SphI-HF® 500 units 2
R0145S XbaI 3,000 units 2
R0146S XhoI 5,000 units 2
NEB # RNA Reagents Size Quantity Available
T2010S Monarch® Total RNA Miniprep Kit 50 preps 2
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each 2
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each 2
M0303S DNase I (RNase-free) 1,000 units 2
T2030S Monarch® RNA Cleanup Kit (10 μg) 10 preps 2
M0525S Quick CIP 1,000 units 2
M0201S T4 Polynucleotide Kinase 500 units 2

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