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UMass Medical School ASC

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
NEB # DNA Modifying Enzymes Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
M0212L Klenow Fragment (3'→5' exo-) 1,000 units M0212L
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M2200L Quick Ligation™ Kit 150 reactions M2200L
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202M T4 DNA Ligase 100,000 units M0202M
M0202S T4 DNA Ligase 20,000 units M0202S
M0202L T4 DNA Ligase 100,000 units M0202L
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R3138S SalI-HF® 2,000 units R3138S
NEB # Markers & Ladders Size Stockroom #
N3232L 1 kb DNA Ladder 1 ml N3232L
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3200S 1 kb Plus DNA Ladder 0.1 ml N3200S
N3200L 1 kb Plus DNA Ladder 0.5 ml N3200L
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N3233S Low Molecular Weight DNA Ladder 0.1 ml N3233S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E3005L Luna® Universal One-Step RT-qPCR Kit 500 reactions E3005L
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions E3006S
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) M3004S
M3003L Luna® Universal qPCR Master Mix 500 rxn (5 x 1 ml) M3003L
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
E3010S LunaScript™ RT SuperMix Kit 25 reactions E3010S
M0541S NEBNext® High-Fidelity 2X PCR Master Mix 50 reactions M0541S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
M0531S Phusion® High-Fidelity PCR Master Mix with HF Buffer 100 reactions (50 µl vol) M0531S
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0493S Q5® Hot Start High-Fidelity DNA Polymerase 100 units M0493S
M0203S T4 DNA Polymerase 150 units M0203S
M0273L Taq DNA Polymerase with Standard Taq Buffer 2,000 units M0273L
M0273S Taq DNA Polymerase with Standard Taq Buffer 400 units M0273S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P7706S Blue Prestained Protein Standard, Broad Range (11-190 kDa) 150 gel lanes P7706S
P7712S Color Prestained Protein Standard, Broad Range (11–245 kDa) 150 gel lanes P7712S
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R3552S AgeI-HF® 300 units R3552S
R0558S AscI 500 units R0558S
R0174S AvrII 100 units R0174S
R3136S BamHI-HF® 10,000 units R3136S
R0144S BglII 2,000 units R0144S
R0197S ClaI 1,000 units R0197S
R3505S EagI-HF® 500 units R3505S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R3142S KpnI-HF® 4,000 units R3142S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3189L NotI-HF® 2,500 units R3189L
R3189S NotI-HF® 500 units R3189S
R0547S PacI 250 units R0547S
R0560S PmeI 500 units R0560S
R3150S PvuI-HF® 500 units R3150S
R3151S PvuII-HF® 5,000 units R3151S
R3156S SacI-HF® 2,000 units R3156S
R0157S SacII 2,000 units R0157S
R3122S ScaI-HF® 1,000 units R3122S
R0141S SmaI 2,000 units R0141S
R0133S SpeI 500 units R0133S
R3133S SpeI-HF® 500 units R3133S
R3182S SphI-HF® 500 units R3182S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
M0290S Alkaline Phosphatase, Calf Intestinal (CIP) 1,000 units M0290S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 µmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 µmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
M0201S T4 Polynucleotide Kinase 500 units M0201S

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