RNA Synthesis

Tools to Take You from Template to Transcript

For almost 50 years, NEB^® has been a world leader in the discovery and production of reagents for the life science industry. New England Biolabs’ enzymology expertise allows us to supply reagents for the synthesis of high-quality RNA – from template generation and transcription, to capping, tailing, and cleanup after synthesis. These products are designed and manufactured by building upon decades of molecular biology experience, so that you can be confident they will work for your application.

In vitro synthesis of single-stranded RNA molecules is a widely used laboratory procedure that is critical to RNA research, as well as to RNA biopharmaceuticals. This technique is versatile in that it allows the researcher to tailor synthesis and introduce modifications to produce a transcript. Downstream applications include biochemical and molecular characterization of RNA for RNA-protein interactions and structural analyses, generation of RNA aptamers, synthesis of functional mRNAs for expression, and generation of small RNAs for alteration of gene expression (e.g., guide RNAs, RNAi). Furthermore, the use of in vitro synthesized RNA has been instrumental in the development of RNA vaccines and CRISPR/Cas9 genome editing tools, generation of pluripotent stem cells, screening of RNA inhibitors, as well as development of RNA amplification-based diagnostics.

High-yield robust reactions require optimization of each reaction component. NEB offers six in vitro RNA synthesis kits, all of which have been optimized to generate reproducible yields of quality RNA. Additionally, individual components can be purchased for in vitro transcription (IVT) and mRNA capping.

NEB’s portfolio of research-grade and GMP-grade* reagents enables bench-scale to commercial-scale mRNA manufacturing. Our optimized HiScribe kits enable convenient in vitro transcription workflows. When it is time to scale up and optimize reaction components, our standalone reagents are readily available in formats matching our GMP-grade offering, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.

Template Generation		In vitro Transcription		RNA Capping		Poly(A) Tailing		RNAPurification
Q5® High-Fidelity DNA Polymerase		HiScribe T7 mRNA Kit with CleanCap® Reagent AG				E. coli Poly(A) Polymerase		Monarch® RNA Cleanup Kit (10 μg)
		HiScribe®; T7 ARCA mRNA Synthesis Kit (with tailing)
dNTP solution mixes		HiScribe T7 ARCA mRNA Synthesis Kit						Monarch RNA Cleanup Kit (50 μg)
BspQI*		HiScribe T7 High Yield RNA Synthesis Components		Faustovirus Capping Enzyme				Monarch RNA Cleanup Kit (500 μg)
				Vaccinia Capping System
DNA Assembly: NEBuilder HiFi DNA Assembly Golden Gate Assembly		HiScribe T7 Quick High Yield RNA Synthesis Kit		mRNA Cap 2′-O-Methyltranferase				Lithium Chloride
		HiScribe SP6 High Yield RNA Synthesis Kit		ARCA and other mRNA cap analogs
		T3 & SP6 RNA Polymerases
		T7 RNA Polymerase
		Hi-T7 RNA Polymerase
		Companion Products						Companion Products
		RNase inhibitor (Murine)						Monarch RNA Cleanup Binding Buffer
		RNase Inhibitor (Human Placental)						Monarch RNA Cleanup Wash Buffer
		Pyrophosphatase, Inorganic (E. coli)						Nuclease-free Water
		Pyrophosphatase, Inorganic (Yeast)
		DNase I (RNase-free)
		DNase I-XT
		NTPs

= available in GMP-grade

*NEB can offer large-scale preparations of restriction enzymes using Recombinant Albumin (BSA-free)