T5 Exonuclease

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0663S     -20       T5 Exonuclease M0663SVIAL -20 1 x 0.1 ml 10,000 units/ml   NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
M0663L     -20       T5 Exonuclease M0663LVIAL -20 1 x 0.5 ml 10,000 units/ml   NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

Application Features

• Degradation of linear ssDNA, dsDNA or nicked plasmid DNA while maintaining supercoiled plasmid DNA.
• Remove denatured DNA isolated during alkaline lysis-based plasmid purification procedure (5).
• Enhance the transfection efficiency of miniprep from plasmid cDNA libraries (6).

Companion Products

• T4 DNA Ligase
• NEBuilder® HiFi DNA Assembly Master Mix
• Nuclease-free Water
• Gibson Assembly® Master Mix

Materials Sold Separately

• NEBuffer 4

1. Sayers, J.R. et al. (1991). Nucleic Acids Res. 19, 4127-4132.
2. Sayers, J.R. et al. (1990). J. of Biol. Chem. 265, 18311-18317.
3. Kaliman, A.V. et al. (1986). FEBS. 195, 61-64.
4. Frenkel, G.D. et al. (1971). J. of Biol. Chem. 246, 4839-4847.
5. Sayers, J.R. et al. (1996). Analytical Biochem. 241, 186-189.
6. Kiss-Toth, E. et al. (2001). Analytical Biochem. 288, 230-232.

1. Protocol for T5 Exonuclease (NEB #M0663)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

1. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
2. Will T5 exonuclease cleave supercoiled dsDNA?
3. How does T5 Exonuclease differ from Exonuclease III (NEB# M0206)?
4. What is the activity of T5 Exonuclease in the NEBuffers?
5. Can T5 Exonuclease be heat inactivated?
6. Can DNA be blunted using T5 Exonuclease?
7. Will T5 Exonuclease work in rCutSmart buffer?
8. What is the difference between T5 Exonuclease (NEB #M0363) and T5 Exonuclease (NEB #M0663)?

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0663S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0663L_v1_10092868
• M0663S_v1_10094098
• M0663S_v1_10095680
• M0663L_v1_10096607
• M0663L_v1_10102112
• M0663L_v1_10105664
• M0663S_v1_10109417
• M0663S_v1_10129015
• M0663L_v2_10140369
• M0663L_v2_10146015
• M0663S_v2_10140371
• M0663L_v2_10155436
• M0663L_v2_10160282
• M0663S_v2_10160286
• M0663S_v2_10164463
• M0663S_v2_10176033
• M0663L_v2_10178415
• M0663S_v2_10191796
• M0663L_v2_10203629
• M0663S_v2_10209949
• M0663S_v2_10228304 copy
• M0663S_v2_10228304
• M0663L_v2_10234103
• M0663L_v2_10244069
• M0663S_v2_10250815
• M0663L_v2_10253495
• M0663S_v2_10239518
• M0663S_v2_10263608
• M0663L_v2_10263633
• M0663S_v2_10272642
• M0663S_v2_10277055
• M0663L_v2_10279637
• M0663S_v2_10287810
• M0663L_v2_10287811
• M0663S_v2_10301424
• M0663L_v2_10308580
• M0663S_v2_10308579
• M0663S_v2_10314215
• M0663L_v2_10316487
• M0663L_v2_10328173
• M0663S_v2_10328171

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T5 Exonuclease
• NEBuffer™ 4

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.