NEBNext Ultra II Q5® Master Mix is the most recent NEBNext formulation of Q5 High Fidelity DNA Polymerase, optimized for amplification of NGS libraries. This hot start formulation is designed for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, and further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.
Optimized for high yields in NGS library amplification
Minimizes GC bias, with superior performance across the GC spectrum
Ultra-high-fidelity amplification with Q5, the highest-fidelity polymerase (1)
Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
Bulk format available: M0544X = 500 reactions in 1 x 12.5 ml bottle
The NEBNext Ultra II Q5® Master Mix is the most recent formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries. This new formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 280-fold higher than that of Taq DNA Polymerase), resulting in ultra-low error rates.
The NEBNext Ultra II Q5 Master Mix is an aptamer-based hot start formulation that allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. NEBNext Ultra II Q5 Master Mix is also included in all of the NEBNext Ultra II library prep kits, for DNA and RNA.
Source An E. coli strain that carries the Q5 High-Fidelity DNA
Reaction Conditions NEBNext Ultra II Q5 Master
Mix, DNA template and 1 μM primers in a total reaction
volume of 50 μl.
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