NEBNext Library Quant DNA Standards are a set of six standards, defining a curve from 100 pM to 0.001 pM, intended for use with the NEBNext Library Quant Master Mix included in the NEBNext Library Quant Kit for Illumina (NEB #E7630).
Additional volume of the same standards included in the NEBNext Library Quant Kit for Illumina
qPCR-based library quant based on either four or six standards
For use with the NEBNext Library Quant Master Mix included in the NEBNext Libarary Quant Kit for Illumina, this product includes six tubes of standards (100 pM to 0.001 pM) providing extra volume for users who require it. Protocols included in the NEB #E7630 product manual detail the process for setting up standard curves using either four or six standards, building flexibility into the workflow.
The NEBNext Library Quant Kit for Illumina offers a streamlined protocol and reduced lot-to-lot variability as compared to similar library quantification kits.
Figure 1: Typical amplification and standard curves for the NEBNext Library Quant Kit
Typical results from the NEBNext Library Quant Kit on a Bio-Rad CFX96 Touch (A) and an Applied Biosystems 7500 Fast real-time qPCR instrument (B). Amplification curves are shown on the left and resulting standard curves on the right. All default settings were used on both platforms. ABI 7500 Fast assay included 1X ROX (Low Concentration) and the ROX normalization.
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