Don’t be biased! The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.
For convenience, a version of this kit is available with SPRIselect beads.
Note: For sheared DNA, we recommend the NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina.
The NEBNext Ultra II FS DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow includes reliable enzymatic DNA fragmentation, requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR.
A separate version of the NEBNext Ultra II FS DNA PCR-free Library Prep Kit is available with sample purification beads.
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