Home Next Generation Sequencing Library Preparation Products NEBNext® LV Unique Dual Index Primers Set 4

NEBNext® LV Unique Dual Index Primers Set 4


Catalog #E3406

NEBNext LV Unique Dual Index Primers Set 4 includes 96 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ v2 and E5hmC-seq™, with Illumina® sequencing. The primers enable incorporation of unique index pairs during library amplification and have been designed to minimize index hopping.

  • Optimized for the low-volume (LV) NEBNext library prep workflows
  • Addresses the “barcode hopping” issue seen with some Illumina sequencing instruments
  • Increases sample identification specificity with unique dual index primer pairs
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  • NEBNext LV Unique Dual Index Primers have been selected and paired for optimal multiplex index incorporation during PCR. Optimized for low-volume (LV) NEBNext workflows, including EM-seq v2 and E5hmC-seq, these primers enable multiplexing and have been designed to minimize index hopping. They are supplied as pairs of premixed i5 and i7 8-base index primers in a 96-well plate that has a pierceable foil seal for easy, single use.

    The index sequences from NEBNext LV Unique Dual Index Primers Set 4 are compatible with and can be combined with the other NEBNext LV Unique Dual Index Primers sets to multiplex up to 480 reactions. Please refer to the relevant NEBNext library prep kit manual for usage instructions. Sample sheets for use with these NEBNext LV Unique Dual Index Primers can be found under "Usage Guidelines" on this product page (see below).

    This product is related to the following categories:
    NEBNext® Multiplex Oligos (Adaptors & Primers) Products,
    Next Generation Sequencing Library Preparation Products

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    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

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      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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