This product is available in Quick-Load Purple format - enjoy sharper bands, no UV shadow, and a lower price.
Size range: 100 bp to 10 kb
Convenient, ready-to-load format
Formulated with bromophenol blue dye
Easy to identify reference bands
Suitable for 250 gel lanes.
Product Information
Quick-Load® 1 kb Plus DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.
Figure 1: Quick-Load 1 kb Plus DNA Ladder visualized
Quick-Load 1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.
Mass values are for 1 µg/lane.
Suggested Load: 5 to 10 µl/lane.
This product is related to the following categories:
Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load 1 kb Plus DNA Ladder per gel lane.
In general, it is recommended to load 1µl per mm of gel lane.
The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
References
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual,(2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 10.51-10.67.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Trademarks
QUICK-LOAD® is a registered trademark of New England Biolabs, Inc. FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.
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