|
POLYMERASE PRODUCTS |
FIDELITY* |
ERROR RATE |
PRODUCT ENDS |
MAX PRODUCT LENGTH** |
EXTENSION TEMPERATURE |
MODIFIED NUCLEOTIDE INCORPORATION*** |
URACIL INCORPORATION |
5´-3´ EXONUCLEASE |
3´-5´ (PROOFREADING) EXONUCLEASE |
|---|---|---|---|---|---|---|---|---|---|
|
Q5 Polymerases |
280X |
<0.44 x 10-6 |
Blunt |
30kb simple, 20kb complex † |
72°C |
5mC, 5hmC, 6mA |
No (except Q5U) |
- |
++++ |
| 20kb simple, 10kb complex | |||||||||
|
Phusion Polymerases |
39-50X |
0.44 x 10-6 |
Blunt |
20kb simple, 10kb complex |
72°C |
5mC, 5hmC |
No |
- |
++++ |
|
OneTaq Polymerases |
2X |
<140 x10-6 |
3´A/blunt |
6kb |
68°C |
5mC, 5hmC, biotin, DIG |
Yes |
+ |
++ |
|
Taq Polymerases |
1X |
2.85 x 10^-4 |
3´A |
5kb |
68°C |
5mC, 5hmC, biotin, DIG |
Yes |
+ |
- |
| Hemo KlenTaq |
nt | nt | 3´A | 2kb | 68°C |
Yes | No | - |
|
| LongAmp® Polymerases |
2X |
3´A/blunt |
30kb |
65°C |
No |
Yes |
++ |
*Fidelity relative to Taq DNA polymerase. We continue to investigate assays to characterize Q5's very low error rate to ensure that we present the most accurate fidelity data possible (Potapov, V, and Ong, J.L. (2017) PloS ONE, 12(1): e0169774).
**Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA and cDNA.
*** For more information, contact Technical Support at info@neb.com.
† Only applicable to the Q5®-XT Hot Start High-Fidelity 2X Master Mix (M2499).
Learn More
Fidelity and error rate
The fidelity of a DNA polymerase is defined by its ability to accurately replicate a template, while error rate is the rate of misincorporation of an incorrectly matched nucleotide. Fidelity is important for applications in which the DNA sequence must be correct after amplification.
To learn more about how fidelity is measured, click here.
Product ends and exonuclease activity
Check out the "Learn More" section on our PCR Product Ends FAQ and our Exonuclease Activity for DNA Polymerases FAQ.
