DNA Amplification

Long Range PCR

Long Range PCR refers to the amplification of DNA lengths that cannot typically be amplified using routine PCR methods or reagents. For simple DNA templates, polymerases optimized for Long Range PCR can amplify up to 30 kb and beyond. For complex, genomic templates, 20 kb is a typical target.

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Protocols for Long Range PCR
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Long Range PCR
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Deoxynucleotide (dNTP) Solution Mix

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Deoxynucleotide (dNTP) Solution Set

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LongAmp® Hot Start Taq 2X Master Mix

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LongAmp® Hot Start Taq DNA Polymerase

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LongAmp® Taq 2X Master Mix

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LongAmp® Taq DNA Polymerase

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LongAmp® Taq PCR Kit

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Phusion GC Buffer Pack

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Phusion HF Buffer Pack

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Phusion High-Fidelity DNA Polymerase

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Phusion High-Fidelity PCR Master Mix with GC Buffer

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Phusion High-Fidelity PCR Master Mix with HF Buffer

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Phusion Hot Start Flex 2X Master Mix

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Phusion Hot Start Flex DNA Polymerase

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Phusion High-Fidelity PCR Kit

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Q5® High-Fidelity 2X Master Mix

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Q5® High-Fidelity DNA Polymerase

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Q5® High-Fidelity PCR Kit

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Q5® Hot Start High-Fidelity 2X Master Mix

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Q5® Hot Start High-Fidelity DNA Polymerase

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Q5® Reaction Buffer Pack

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Q5®-XT Hot Start High-Fidelity 2X Master Mix

Videos

  • Tips for Amplifying Large Amplicons

    Do you need to optimize your PCR of large amplicons? Becky shares some tips for choosing the right polymerase and cycler conditions, as well as optimal handing methods, to get the most out of those long templates!