Monarch^® DNase I, Lyophilized

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T2104S     25       Monarch® DNase I, Lyophilized T2104-1 25 1 x 2,200 units Not Applicable   Monarch® DNase I Reaction Buffer T2105-1 25 1 x 4.6 ml Not Applicable

Companion Products

• Monarch^® Spin RNA Isolation Kit Mini

1. Protocol for Rehydration of Monarch DNase I, Lyophilized (NEB #T2104)
2. Protocol for DNase digestion reaction in solution using Monarch DNase I, Lyophilized (NEB #T2104)
3. Protocol for on-column DNase reaction using Monarch DNase I, Lyophilized (NEB #T2104) and Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)

1. What is the difference between Monarch DNase I, Lyophilized (NEB #T2104) and DNase I, (RNase-free) (NEB #M0303)?
2. How do I store the Monarch DNase I, Lyophilized (NEB #T2104) upon receiving it?
3. How do I store Monarch DNase I, Lyophilized (NEB #T2104) after rehydration?
4. How many freeze-thaws can the rehydrated Monarch DNase I, Lyophilized (NEB #T2104) tolerate?
5. What volume should I rehydrate in to use with Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)?
6. What volume should I rehydrate in to make it equivalent to NEB’s liquid DNase product NEB #M0303?
7. I want a concentrated form of DNase that is higher than the typical range. What is the minimum volume for rehydration of Monarch DNase I, Lyophilized (NEB #T2104)?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T2104S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2104S_v1_10294958
• T2104S_v1_10295270
• T2104S_v1_10295280

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® DNase I, Lyophilized
• Monarch^® DNase I Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.