Monarch® kits excel against leading suppliers in performance and sustainability
Monarch kits offer value without compromise
Monarch is your solution for nucleic acid purification, enabling quick and easy purification of high-quality DNA and RNA suitable for a range of downstream applications. Our user-friendly protocols and optimized buffer systems let you recover pure, intact DNA and RNA in minutes. Monarch products allow you more time to focus on the experiments that will drive your research forward.
Our kits enable fast, easy workflows without compromising on quality or value. Monarch kits consistently perform as well as or better than leading suppliers. See our performance data and find out what customers have to say when it comes to DNA cleanup and gel extraction, plasmid purification, genomic DNA purification, and total RNA extraction and purification.
Monarch Kits for DNA Gel Extraction & PCR Cleanup
“In comparison, the yield of DNA I'm getting from the Monarch kit is 5 to 10 times greater than with the Qiagen. Also, the quality of DNA is also better (based on Nanodrop absorbance curve). Finally, the Monarch kit is easier to use.” – Researcher, Yale University
The Monarch Spin DNA Gel Extraction Kit consistently recovers DNA as well as or better than the leading supplier, and is more concentrated for greater downstream utility
Using the Monarch Spin DNA Gel Extraction Kit, DNA concentrations and recovery are equal to or higher than a leading suppliers' kits. 2 μg of 3 kb fragment were resolved on a 1.2 % w/v TBE agarose gel, excised and processed with recommended protocols and elution volumes. Concentrations of DNA were measured using a Trinean DropSense 16 and percent recovery calculations are based on the eluted DNA concentration and elution volume used. “I have been struggling to isolate enough product from the MinElute PCR cleanup and gel extraction kit for my PCR cloning project to be successful. We tried the Monarch PCR cleanup kit as a last-ditch effort and our ligation reaction was successful on the first try!” – Assistant Specialist, University of California Irvine
Monarch Spin PCR & DNA Cleanup Kit (5 μg) consistently produces more concentrated DNA, with equivalent or better DNA recovery and purity compared to the leading supplier
Using the Monarch Spin PCR & DNA Cleanup Kit (5 μg), DNA concentrations and recovery are equal to or higher than a leading suppliers' kits. Preps were performed according to recommended protocols and elution volumes using 5 μg of 3 kb linear DNA fragment as an input. Concentrations of DNA were measured using a Trinean DropSense 16 and percent recovery calculations are based on the eluted DNA concentration and elution volume used.
Monarch Kits for Plasmid Purification
“We have been using Qiagen for a very long time but when compared to the Monarch kit, we were surprised at how much more product we were able to collect. Great kits, easy to use protocols!! A+” – Researcher, Yale University
Monarch Spin Plasmid Miniprep Kit consistently produces more concentrated plasmid DNA with equivalent or better yield and purity compared to the leading supplier
DNA concentration, yield and purity are higher with Monarch Spin Plasmid Miniprep Kit than leading supplier’s kit, across different plasmids. Preps were performed according to recommended protocols using ~1 ml (OD600 = 3) aliquot for pUC19 and pHT01 and ~2 ml (OD600 = 6) aliquot for Rluc and pXba of the same overnight cultures. Concentrations of plasmid were measured using a Trinean DropSense 16.
Monarch Kits for Genomic DNA Purification
“This kit yielded the highest purity DNA I have ever seen from a commercial spin column kit.” – Stephen, Professor, Lake Superior State University
The Monarch Spin gDNA Extraction Kit provides excellent yields with tissues that are problematic for other commercial kits
Duplicate 10 mg samples of RNAlater®-stabilized rat tissue were cut to small pieces and subsequently lysed and purified according to the protocols provided with each kit. Optional RNase A steps were included. Elution was carried out with 100 µl elution buffer provided in the respective kits. Yields displayed are averages of the duplicate samples, and represent the genomic DNA yield after correcting for the RNA content as determined by LC-MS. Results indicate that the Monarch Spin gDNA Purification Kit provides excellent yields for a wide range of tissues.
Monarch Kits for Total RNA Extraction & Purification
Sample Type: Gram Positive Bacteria (compared to: Qiagen RNeasy Mini)
“I've been using the RNeasy kit for some time, but I've been unable to have much luck. It seems geared toward eukaryotes and I've been working with Gram Positive prokaryotes… I tried the NEB [Total] RNA Miniprep Kit and it was much more straightforward. I also got excellent results the first time I used it.” – Kyle, Postdoctoral Researcher, University of CA, Berkeley
The Monarch Spin RNA Isolation Kit (Mini) successfully purifies small RNAs below 200 nucleotides, enabling a more faithful representation of the total RNA pool compared to a leading supplier
RNA was extracted from 10 mg mouse brain tissues using Monarch Spin RNA Isolation Kit (Mini) and a comparable kit from Supplier Q. To visualize the isolation of small RNA species, the extracted RNA was resolved using the Agilent Small RNA chip on a Bioanalyzer 2100 according to manufacturer's instructions. Gel traces of RNA isolated from the two kits are shown (A), with representative electropherograms showing the small RNA size profiles (B).
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