Application Notes
Filter by Application
79 Results
Accelerating DNA Construction to Protein Expression: A Rapid 1-Day Workflow Using NEBridge® Golden Gate Assembly
Last Updated August 2023
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Protein Expression and Purification
A one-day in vitro workflow of Golden Gate Assembly, RCA, and Cell-free protein expression for high-throughput screening, generating custom protein variants.
Improving NGS library performance with lower input amounts using the NEBNext Ultra II RNA Library Prep Kit for Illumina (non-directional)
Last Updated July 2023
Application: NGS Sample Prep and Target Enrichment
This technical note describes non-directional RNA library preparation using poly(A) mRNA enrichment and NEBNext® Ultra II RNA Library Prep Kit for Illumina.
Improving NGS library performance with lower input amounts using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (non-directional)
Last Updated July 2023
Application: NGS Sample Prep and Target Enrichment
This technical note describes non-directional RNA library preparation using a rRNA depletion workflow and NEBNext Ultra II RNA Library Prep Kit for Illumina.
Scaling down to scale up Miniaturizing cell free protein synthesis reactions with the Echo 525 Acoustic Liquid Handler
Last Updated July 2023
Application: Protein Expression and Purification
This application note defines the input effects, volume constraints and reproducibility of miniature NEBExpress and PURExpress reactions assembled with the Echo 525 Acoustic Liquid Handler.
NEBNext® Enzymatic Methyl-seq (EM-seq™)
Last Updated July 2023
Application: Epigenetics and Cellular Analysis, Glycobiology and Protein Analysis
This technical note describes a new high-performance, enzyme-based methodology developed at NEB that provides an alternative to bisulfite sequencing for methylome analysis.
Improved methods for site-directed mutagenesis using NEBuilder® HiFi DNA Assembly Master Mix
Last Updated May 2023
Application: Cloning and Synthetic Biology
This application note describes the use of the NEBuilder® HiFi DNA Assembly Master Mix for the generation of single or multiple site-directed mutations.
Nanoliter Scale DNA Assembly Utilizing the NEBuilder® HiFi Cloning Kit with the Labcyte® Echo® 525 Liquid Handler
Last Updated May 2023
Application: Cloning and Synthetic Biology
This application note describes the use of the Labcyte Echo 525 Liquid Handler to miniaturize reaction volumes for two- and five-piece assemblies.
Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments
Last Updated May 2023
Application: Cloning and Synthetic Biology
This application note describes an NEB protocol to construct an sgRNA-Cas9 expression vector with ssDNA oligos, a digested vector and NEBuilder® HiFi DNA Assembly Master Mix.
Improved methods for site-directed mutagenesis using Gibson Assembly® Master Mix
Last Updated May 2023
Application: Cloning and Synthetic Biology, NGS Sample Prep and Target Enrichment
This technical note outlines how the Gibson Assembly Master Mix from NEB can reduce time and cost when performing site-directed mutagenesis.
Increased transcription detection with the NEBNext® Single Cell/Low Input RNA Library Prep Kit
Last Updated December 2022
Application: NGS Sample Prep and Target Enrichment
This technical note demonstrates use of the NEBNext Single Cell/Low Input RNA Library Prep Kit for high quality library prep from single cells or low inputs.
High-yield, Scalable Library Preparation with the NEBNext® Ultra™ II FS DNA Library Prep Kit
Last Updated December 2022
Application: NGS Sample Prep and Target Enrichment
This technical note describes DNA library preparation with enzymatic DNA fragmentation using the NEBNext Ultra II FS DNA Library Prep Kit.
Automated size selection of NEBNext® Small RNA libraries with the Sage Pippin Prep™
Last Updated November 2022
Application: NGS Sample Prep and Target Enrichment
This technical note outlines the use of Pippin Prep beads for size selection of NEBNext Small RNA libraries.
Monarch® HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies® sequencing of malaria parasites
Last Updated November 2022
Application: NGS Sample Prep and Target Enrichment
In this customer-generated technical note, see how the Monarch HMW DNA Extraction Kit was used to extract DNA from Plasmodium and improved nanopore sequencing results for de novo genome assembly.
Obtain superior NGS library performance with lower input amounts using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Last Updated November 2022
Application: NGS Sample Prep and Target Enrichment
This technical note describes RNA library preparation using a rRNA depletion workflow and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.
Multiplex real-time PCR detection of monkeypox virus using Luna® qPCR Reagents
Last Updated October 2022
Application: DNA Amplification PCR and qPCR
This app note describes how Luna qPCR reagents can be used to detect the monkeypox virus with high sensitivity and specificity in single and multiplex assays.
Optimized conditions for the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay using Luna One-Step RT-qPCR Reagents
Last Updated August 2022
Application: DNA Amplification PCR and qPCR
This application note describes reaction conditions that improved the consistency and sensitivity of FluA, FluB, and SARS-CoV-2 multiplexed reactions.
Detection of the Omicron variant mutation at position 26,270 in the SARS-CoV-2 E gene using the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit
Last Updated August 2022
Application: DNA Amplification PCR and qPCR
This application note describes the impact of a specific Omicron mutation on the sensitivity of the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit.
Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
Last Updated July 2022
Application: Epigenetics and Cellular Analysis, Glycobiology and Protein Analysis
This application note provides protocols for labeling of E. coli expressed SNAP-tag fusion proteins.
Simultaneous Fluorescent Labeling of Proteins in Living Cells
Last Updated July 2022
Application: Epigenetics and Cellular Analysis, Glycobiology and Protein Analysis
This application note describes the simultaneous fluorescent labeling of proteins in living cells using NEB's SNAP-tag® and CLIP-tag™.
Enzymatic PCR cleanup using Exonuclease I and Shrimp Alkaline Phosphatase
Last Updated July 2022
Application: Cloning and Synthetic Biology
This application note describes PCR cleanup using Exonuclease I to degrade residual primers, and Shrimp Alkaline Phosphatase to dephosphorylate remaining dNTPs.
Intein-Mediated Protein Ligation IPL and Labeling with the IMPACT™ Kit
Last Updated July 2022
Application: Glycobiology and Protein Analysis, Protein Expression and Purification
This application note provides protocols for intein-mediated protein ligation (IPL) and protein labeling using NEB's IMPACT Kit.
Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix
Last Updated July 2022
Application: Glycobiology and Protein Analysis
This application note describes the enzymatic removal of glycans from a protein using PNGase F, or the Protein Deglycosylation Mix.
Ph.D.™ Peptide Display Cloning System
Last Updated July 2022
Application: Glycobiology and Protein Analysis
This application note provides protocols for cloning a library of short peptides into M13KE phage using the Ph.D. Peptide Display Cloning System.
Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes
Last Updated July 2022
Application: Cloning and Synthetic Biology
This application note describes the use of NEB's OneTaq® Quick-Load® Master Mixes in colony PCR from multiple E. coli strains.
A faster workflow for the assessment of genomic loci in mice using a novel HMW DNA extraction technology upstream of Cas9 targeted sequencing
Last Updated May 2022
Application: Genome Editing
This technical note reviews how scientists at The Jackson Laboratory were able to significantly reduce their workflow time for assessment of transgene insert sites in mouse lines using the novel glass-bead based Monarch® HMW DNA Extraction Kit upstream of their Cas9 targeted sequencing workflow.
Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
Last Updated March 2022
Application: Protein Expression and Purification
This application note describes the advantages of using PURExpress cell-free in vitro transcription/ translation system for high-throughput screening.
Enhancing Transformation Efficiency
Last Updated March 2022
Application: Cloning and Synthetic Biology, Protein Expression and Purification
This application note provides protocols and tips for enhancing transformation efficiency of competent E. coli cell lines #C2523, C2925, C2984, C2987, C2988, C2992 and C3019.
Fast & efficient isolation of phage genomic DNA using the Monarch HMW DNA Extraction Kits
Last Updated March 2022
Application: Cloning and Synthetic Biology
This technical note discusses rapid bacteriophage DNA extraction using a novel glass bead method.
Key considerations for optimal lyophilized reagent development
Last Updated February 2022
Application: DNA Amplification PCR and qPCR
This white paper details the process and considerations of lyophilized reagent development for use in research, applied and diagnostics markets.
SNAPf -based pulse labeling for analysis of protein turnover in living cells
Last Updated February 2022
Application: Epigenetics and Cellular Analysis, Glycobiology and Protein Analysis
This application note describes the labeling of fusion proteins using an improved version of SNAP-tag, SNAPf.
Improved library quantitation for a broad range of library types using the NEBNext® Library Quant Kit for Illumina®
Last Updated January 2022
Application: NGS Sample Prep and Target Enrichment
This technical note describes the effectiveness of the NEBNext Library Quant Kit for a broad range of library types and sizes.
Optimizing rapid isothermal workflow for SARS-CoV-2 with WarmStart® LAMP with UDG
Last Updated December 2021
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Epigenetics and Cellular Analysis, Genome Editing, Glycobiology and Protein Analysis, NGS Sample Prep and Target Enrichment, Protein Expression and Purification, RNA Analysis
This application note describes the use of WarmStart Multi-purpose LAMP/RT LAMP 2X Master Mix (with UDG) for rapid and sensitive detection of SARS-CoV-2 RNA.
Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
Last Updated December 2021
Application: Protein Expression and Purification
This application note describes the use of PURExpress and PDBE to express active proteins in vivo in Shuffle™ competent cells.
Co-expression of Multiple Proteins in Kluyveromyces lactis
Last Updated December 2021
Application: Protein Expression and Purification
This application note describes an easy protocol for the construction of K. lactis strains that co-express multiple heterologous proteins.
Protein Expression with T7 Express Strains
Last Updated December 2021
Application: Glycobiology and Protein Analysis, Protein Expression and Purification
This application note provides protocols for T7 protein expression with NEB's T7 Express Competent E. coli.
NEBExpress® Cell-free E. coli Protein Synthesis System
Last Updated December 2021
Application: Protein Expression and Purification
This technical note describes NEB’s new, versatile, lysate-based, cell-free protein synthesis system for use with circular or linear DNA, or mRNA templates.
Transformation of SHuffle® Competent Cell Strains
Last Updated December 2021
Application: Glycobiology and Protein Analysis, Protein Expression and Purification
This application note describes the use of Shuffle Comptetent E.coli Cells to enable the folding of recombinantly expressed proteins in vivo.
Automated library preparation using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® on the epMotion® 5075t
Last Updated October 2021
Application: NGS Sample Prep and Target Enrichment
This application note describes a robust automated workflow for the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina on the epMotion 5075t.
Protocol for using Recombinant Cas9 Nuclease to Assess Locus Modification in Genome Editing Experiments
Last Updated September 2021
Application: Cloning and Synthetic Biology
This application note describes sensitive in vitro digestion of PCR amplicons using Cas9 Nuclease for detecting indels in genome editing experiments.
A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers
Last Updated August 2021
Application: Cloning and Synthetic Biology, RNA Analysis
This technical note provides detailed explanations and guidance on how to interpret and use MVS measurements, with a focus on samples purified using silica spin columns.
Optimized Integration of New England Biolabs® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments
Last Updated August 2021
Application: DNA Amplification PCR and qPCR
This app note details the optimized settings needed to analyze single and multiplex LAMP reactions on Axxin instruments.
Analysis of 5-methylcytosine and 5-hydroxymethylcytosine Levels in Human Brain and Liver DNA Samples using the EpiMark® 5-hmC and 5-mC Analysis Kit
Last Updated August 2021
Application: Epigenetics and Cellular Analysis
This application note demonstrates the use of the EpiMark 5-hmC and 5-mC Analysis Kit on different tissue samples.
Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry
Last Updated July 2021
Application: Glycobiology and Protein Analysis
This application note describes the use of Protein Deglycosylation Mix II with PNGase F to show the presence of N- or O-glycans in the protein of interest.
Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit
Last Updated July 2021
Application: Glycobiology and Protein Analysis
This application note describes the NEB N-glycan Sequencing Kit for the characterization of glycans on Fc domains of therapeutic antibodies and fusion proteins.
Characterization of Glycans from Erbitux®, Rituxan® and Enbrel® using PNGase F (Glycerol-free), Recombinant
Last Updated July 2021
Application: Glycobiology and Protein Analysis
This application note describes the characterization of glycans from Erbitux, Rituxan and Enbrel using PNGase F (Glycerol-free), Recombinant.
Unbiased and fast IgG deglycosylation for accurate N-glycan analysis using Rapid™ PNGase F
Last Updated July 2021
Application: Glycobiology and Protein Analysis
This application note describes deglycosylation in minutes using Rapid PNGase F, enabling monitoring of antibody glycosylation, specifically N-glycan profiles.
Fully Automated NEBNext® Direct Genotyping Solution using Beckman Coulter® Life Sciences Biomek i7 Workstation
Last Updated July 2021
Application: NGS Sample Prep and Target Enrichment
This application note describes the automation of NEBNext Direct Genotyping Solution using Beckman Coulter Biomek i7 Hybrid workstation for cost-effective, high-throughput, plant and animal applications.
Automated NEBNext® Ultra™ II DNA Library Preparation for Illumina®
Last Updated July 2021
Application: NGS Sample Prep and Target Enrichment
This application note describes an automated NEBNext Ultra II DNA library prep workflow for Illumina, with end repair and dA-tailing steps, on the Freedom EVO NGS workstation.
Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina® on the Beckman Coulter® Biomek FXP Automated Liquid Handler
Last Updated July 2021
Application: NGS Sample Prep and Target Enrichment
This technical note describes automation of DNA library construction on the Beckman Coulter Biomek FXP Dual Arm Multi-Channel 96 and Span-8 automated liquid handler.
Automated Directional RNA-seq Library Preparation using the Biomek FXP Liquid Handler
Last Updated July 2021
Application: NGS Sample Prep and Target Enrichment
This technical note describes the automation of the NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® on the Beckman Coulter® Biomek FXP automated liquid handler.
GUIDElines for optimization of Tth Argonaute (TtAgo) reactions
Last Updated July 2021
This technical note provides useful tips to help with designing guides for Tth Argonaute reactions.
Using Glycosidases to Remove, Trim, or Modify Glycans on Therapeutic Proteins
Last Updated July 2021
Application: Glycobiology and Protein Analysis
This application note describes the use of glycosidases on therapeutic proteins to remove glycans for analysis, trim glycans for sequencing, or modify glycans in glycoengineering.
E. coli Lemo21 DE3-A T7 RNA Polymerase-based protein overexpression platform for routine and difficult targets
Last Updated July 2021
Application: Glycobiology and Protein Analysis, Protein Expression and Purification
This application note describes the expression of difficult proteins using the T7 strain, Lemo21(DE3).
Biomek i-Series Automated New England Biolabs NEBNext® Ultra™ II DNA Library Prep Kit
Last Updated July 2021
Application: NGS Sample Prep and Target Enrichment
This application note describes the automation of the NEBNext Ultra II DNA Library Prep Kit for the Beckman Biomek i-Series.
Bridging dsDNA with a ssDNA Oligo and NEBuilder® HiFi DNA Assembly to create an sgRNA-Cas9 Expression Vector
Last Updated July 2021
Application: Cloning and Synthetic Biology
This application note describes the construction of an sgRNA-Cas9 expression vector using ssDNA oligos and NEBuilder® HiFi DNA Assembly Master Mix.
Improved method for assembly of linear yeast expression cassettes using NEBuilder® HiFi DNA Assembly Master Mix
Last Updated July 2021
Application: Cloning and Synthetic Biology
This application note describes in vitro DNA assembly of expression cassettes using NEBuilder® HiFi DNA Assembly Mix and NEB's K. lactis Protein Expression Kit.
Improved Library Preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
Last Updated July 2021
Application: NGS Sample Prep and Target Enrichment
This technical note on DNA library preparation addresses lower input amounts and challenging sample types.
Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit
Last Updated July 2021
Application: RNA Analysis
This application note describes how Monarch kits can be used for effective viral RNA extraction for SARS-CoV-2 research, including leveraging the QIAcube® and KingFisher™ Flex automation platforms.
Rapid Analysis of Genome Editing Efficiency using PCR Amplicons processed by the Exo-CIP™ Rapid PCR Cleanup Kit followed by Sanger sequencing
Last Updated July 2021
Application: Genome Editing
This application note describes the analysis of genome editing efficiency by using the Exo-CIP™ Rapid PCR Cleanup Kit for PCR cleanup upstream of Sanger sequencing.
Using Exonuclease V (RecBCD) to Eliminate
Residual Genomic DNA When Purifying Low Copy
Plasmids with the Monarch® Plasmid Miniprep Kit
Last Updated July 2021
Application: Cloning and Synthetic Biology
This application note describes the use of Exonuclease V to degrade contaminating gDNA during plasmid miniprep of low copy plasmids.
Breaking through the Limitations of Golden Gate Assembly
Last Updated July 2021
Application: Cloning and Synthetic Biology
Ligase fidelity data and BsaI-HF®v2 provide 20+ fragment Golden Gate assemblies that exhibit high efficiencies, >90% accuracy and low backgrounds.
Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using the Blunt/TA Ligase Master Mix
Last Updated July 2021
Application: Cloning and Synthetic Biology
This application note describes the use of the Blunt/TA Ligase Master Mix for adaptor ligation to dA tailed dsDNA fragments.
Selective Depletion of Abundant RNAs to Enable Transcriptome Analysis of Low-input and Highly-degraded RNA from FFPE Breast Cancer Samples
Last Updated June 2021
Application: NGS Sample Prep and Target Enrichment
Application note describing selective depletion of abundant RNA from human, mouse and rat, as well as degraded and FFPE breast tumor biopsy RNA samples.
Remove iT PNGase F Effective Release and Recovery of Neutral and Sialylated N glycans
Last Updated June 2021
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Epigenetics and Cellular Analysis, Genome Editing, Glycobiology and Protein Analysis, NGS Sample Prep and Target Enrichment, Protein Expression and Purification, RNA Analysis
This application note describes Remove-iT® PNGase F, a new version of PNGase F, that can be easily removed after deglycosylation using chitin magnetic beads.
Proteomics Fast and Efficient Antibody Deglycosylation using Rapid PNGase F
Last Updated June 2021
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Epigenetics and Cellular Analysis, Genome Editing, Glycobiology and Protein Analysis, NGS Sample Prep and Target Enrichment, Protein Expression and Purification, RNA Analysis
This application note describes complete removal of N-glycans from antibodies using Rapid™ PNGase F, overcoming lengthy incubation steps needed using PNGase F.
Probe-based qPCR Probe Compatibility and Multiplexing with Luna Universal Probe qPCR Master Mix
Last Updated June 2021
Application: DNA Amplification PCR and qPCR
This application note describes qPCR multiplexing using Luna® Universal Probe qPCR Master Mix for a high performance reaction.
Multiplex PCR using
Q5 High-Fidelity DNA Polymerase
Last Updated June 2021
Application: DNA Amplification PCR and qPCR
This application note describes the use of NEB's Q5® High-Fidelity DNA Polymerase in multiplex PCR.
Joining of Difficult to Ligate dsDNA Fragments with Blunt TA Ligase Master Mix
Last Updated June 2021
Application: Cloning and Synthetic Biology
This application note describes the use of NEB's Blunt/TA Ligase Master Mix for ligating difficult overhangs and blunt ends.
A Fast One-Step Digestion of DNA or RNA for Global Detection and Characterization of Nucleotide Modifications
Last Updated June 2021
A Fast One-step Digestion of DNA or RNA for Global Detection and Characterization of Nucleotide Modifications
DNA Methylation and Restriction Digests
Last Updated June 2021
Application: Epigenetics and Cellular Analysis
This application note describes how DNA methylation can interfere with restriction digests and how NEB's dam-/dcm- or dam- Competent E.coli cells overcome this.
The Luna 4X RT-qPCR Mix and Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit enable high throughput, automated detection workflows
Last Updated June 2021
Application: DNA Amplification PCR and qPCR
This note describes features of the Luna 4X RT-qPCR mix and multiplex assay kit that enable high-throughput, automated SARS-CoV-2 detection workflows.
Quick Ligation Kit
Last Updated June 2021
Application: Cloning and Synthetic Biology
This application note provides protocols for ligation of a variety of cohesive or blunt end DNA using NEB's Quick Ligation™ Kit.
Primer Monitor: an online tool to track SARS-CoV-2 variants that may impact primers used in diagnostic assays
Last Updated June 2021
Application: DNA Amplification PCR and qPCR
The Primer Monitor tool can inform SARS-CoV-2 variant studies on primer/probe binding in COVID-19 diagnostic assays, as described in this white paper.
Glycan Analysis of Murine IgG2a by Enzymatic Digestion with PNGase F and Trypsin, Followed by Mass Spectrometric Analysis
Last Updated June 2021
Application: Glycobiology and Protein Analysis
This application note describes the use of PNGase F, trypsin and mass spectroscopy for the release and characterization of glycans on Murine IgG2a.
Glycan Analysis of Murine IgG by Enzymatic Digestion with Endo S and PNGase F Followed by Mass Spectrometric Analysis
Last Updated June 2021
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Epigenetics and Cellular Analysis, Genome Editing, Glycobiology and Protein Analysis, NGS Sample Prep and Target Enrichment, Protein Expression and Purification, RNA Analysis
This application note describes the more robust enzymatic removal of glycans on native Murine IgG using Endo S compared to incomplete digestion using PNGase F.
Enzymatic Deglycosylation of a Protein Containing Core 1 O-glycans with O-Glycosidase
Last Updated June 2021
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Epigenetics and Cellular Analysis, Genome Editing, Glycobiology and Protein Analysis, NGS Sample Prep and Target Enrichment, Protein Expression and Purification, RNA Analysis
This application note describes the use of a recombinant O-Glycosidase that can cleave core 1 O-linked disaccharides (5) and immature Tn core (GalNAc).
Removal of terminal galactose from a glycoprotein containing tri and tetraantennary N linked sugars with 1 3 6 Galactosidase
Last Updated June 2021
Application: Cloning and Synthetic Biology, DNA Amplification PCR and qPCR, Epigenetics and Cellular Analysis, Genome Editing, Glycobiology and Protein Analysis, NGS Sample Prep and Target Enrichment, Protein Expression and Purification, RNA Analysis
This application note describes the use of an a1-3,6 Galactosidase to remove terminal galactose residues from the tri- and tetra- antennary N-glycoprotein.
Facilitating Detection of SARS-CoV-2 Directly from Patient Samples: Precursor Studies with RT-qPCR and Colorimetric RT-LAMP Reagents
Last Updated May 2021
This paper evaluates the use of NEB RT-qPCR and colorimetric RT-LAMP reagents on unpurified RNA samples through the use of synthetic SARS-Cov-2 viral RNA.
High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB Luna Reagents
Last Updated April 2021
Application: DNA Amplification PCR and qPCR
This article outlines the use of NEB Luna qPCR and RT-qPCR reagents with the Beckman Coulter Echo Acoustic liquid handler.
