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89 Results

Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production

Last Updated May 2025

Application: RNA Analysis

This technical note includes recommendations for scaling of workflows utilizing both the HiScribe^® line of T7 and SP6 RNA Polymerase-based in vitro transcription kits, as well as individual enzymes (T7 RNA Polymerase, Inorganic Pyrophosphatase (E. coli) and RNase Inhibitor, Murine).

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89 Results

Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production

LAMP Primer Design using the NEB LAMP Primer Design Tool: Critical Considerations for Assay Robustness, Speed and Sensitivity

Probe-based qPCR Probe Compatibility and Multiplexing with Luna Universal Probe qPCR Master Mix

Monarch® HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies® sequencing of malaria parasites

Facilitating Purification and Detection of Viral Nucleic Acids from Milk

Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit

A faster workflow for the assessment of genomic loci in mice using a novel HMW DNA extraction technology upstream of Cas9 targeted sequencing

High-throughput mass spectrometry-based enzyme screening enabled by streptavidin magnetic beads

Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads

Selective Depletion of Abundant RNAs to Enable Transcriptome Analysis of Low-input and Highly-degraded RNA from FFPE Breast Cancer Samples

High-yield, Scalable Library Preparation with the NEBNext® Ultra™ II FS DNA Library Prep Kit

Automated size selection of NEBNext® Small RNA libraries with the Sage Pippin Prep™

Improved library quantitation for a broad range of library types using the NEBNext® Library Quant Kit for Illumina®

Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina® on the Beckman Coulter® Biomek FXP Automated Liquid Handler

Automated NEBNext® Ultra™ II DNA Library Preparation for Illumina®

Improved Library Preparation with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

A Fast One-Step Digestion of DNA or RNA for Global Detection and Characterization of Nucleotide Modifications

Accelerating DNA Construction to Protein Expression: A Rapid 1-Day Workflow Using NEBridge® Golden Gate Assembly

NEBNext® Enzymatic Methyl-seq (EM-seq™)

Multiplex real-time PCR detection of monkeypox virus using Luna® qPCR Reagents

DNA Methylation and Restriction Digests

Automated Directional RNA-seq Library Preparation using the Biomek FXP Liquid Handler

Automated library preparation using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® on the epMotion® 5075t

Obtain superior NGS library performance with lower input amounts using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®

Improving NGS library performance with lower input amounts using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (non-directional)

Improving NGS library performance with lower input amounts using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (non-directional)

Fully Automated NEBNext® Direct Genotyping Solution using Beckman Coulter® Life Sciences Biomek i7 Workstation

Biomek i-Series Automated New England Biolabs NEBNext® Ultra™ II DNA Library Prep Kit

Increased transcription detection with the NEBNext® Single Cell/Low Input RNA Library Prep Kit

A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using the Blunt/TA Ligase Master Mix

Generating Oligonucleotide Probes using Immobilized T4 DNA Ligase minimizes sample loss and saves time

Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit

Improved Methods for SDM using Gibson Assembly Master Mix

Determining Efficiency of On-Target CRISPR Cas9 Genome Editing Using the NEB® EnGen® Mutation Detection Kit on LabChip Gel Xpress

DNA Shuffling using NEBridge® Golden Gate Assembly for Protein Engineering

Comprehensive structural and positional profiling of intact complex O-glycans in biologic drugs using O-Glycoprotease (IMPa)

Characterization of Glycans from Erbitux®, Rituxan® and Enbrel® using PNGase F (Glycerol-free), Recombinant

Proteomics Fast and Efficient Antibody Deglycosylation using Rapid PNGase F

Unbiased and fast IgG deglycosylation for accurate N-glycan analysis using Rapid™ PNGase F

Glycan Analysis of Murine IgG by Enzymatic Digestion with Endo S and PNGase F Followed by Mass Spectrometric Analysis

Nanoliter Scale DNA Assembly Utilizing the NEBuilder® HiFi Cloning Kit with the Labcyte® Echo® 525 Liquid Handler

Removal of terminal galactose from a glycoprotein containing tri- and tetra-antennary N-linked sugars with α1-3 6 Galactosidase

Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments

Protocol for using Recombinant Cas9 Nuclease to Assess Locus Modification in Genome Editing Experiments

Bridging dsDNA with a ssDNA Oligo and NEBuilder® HiFi DNA Assembly to create an sgRNA-Cas9 Expression Vector

Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes

Improved methods for site-directed mutagenesis using Gibson Assembly® Master Mix

Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants

Multiplex PCR using Q5® High-Fidelity DNA Polymerase

Protein Expression with T7 Express Strains

Transformation of SHuffle® Competent Cell Strains

Simultaneous Fluorescent Labeling of Proteins in Living Cells

Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins

Enhancing Transformation Efficiency

Scaling down to scale up Miniaturizing cell free protein synthesis reactions with the Echo 525 Acoustic Liquid Handler

Enzymatic Deglycosylation of a Protein Containing Core 1 O-glycans with O-Glycosidase

Remove iT PNGase F Effective Release and Recovery of Neutral and Sialylated N glycans

Optimizing rapid isothermal workflow for SARS-CoV-2 with WarmStart® LAMP with UDG

Improved methods for site-directed mutagenesis using NEBuilder® HiFi DNA Assembly Master Mix

Optimized conditions for the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay using Luna One-Step RT-qPCR Reagents

Detection of the Omicron variant mutation at position 26,270 in the SARS-CoV-2 E gene using the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit

Enzymatic PCR cleanup using Exonuclease I and Shrimp Alkaline Phosphatase

Intein-Mediated Protein Ligation IPL and Labeling with the IMPACT™ Kit

Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix

Ph.D.™ Peptide Display Cloning System

Fast & efficient isolation of phage genomic DNA using the Monarch HMW DNA Extraction Kits

Key considerations for optimal lyophilized reagent development

SNAPf -based pulse labeling for analysis of protein turnover in living cells

Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.

Co-expression of Multiple Proteins in Kluyveromyces lactis

NEBExpress® Cell-free E. coli Protein Synthesis System

Optimized Integration of New England Biolabs® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments

Analysis of 5-methylcytosine and 5-hydroxymethylcytosine Levels in Human Brain and Liver DNA Samples using the EpiMark® 5-hmC and 5-mC Analysis Kit

Analysis of a Fusion Protein using the Protein Deglycosylation Mix II and Mass Spectrometry

Detailed Characterization of Antibody Glycan Structure using the N-Glycan Sequencing Kit

GUIDElines for optimization of Tth Argonaute (TtAgo) reactions

Using Glycosidases to Remove, Trim, or Modify Glycans on Therapeutic Proteins

Monarch^® HMW DNA Extraction Kit improves sample preparation for Oxford Nanopore Technologies^® sequencing of malaria parasites

Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch^® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit

Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress^® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads

High-yield, Scalable Library Preparation with the NEBNext^® Ultra™ II FS DNA Library Prep Kit

Automated size selection of NEBNext^® Small RNA libraries with the Sage Pippin Prep™

Automated DNA Library Construction Using the NEBNext Ultra DNA Library Prep Kit for Illumina^® on the Beckman Coulter^® Biomek FX^P Automated Liquid Handler

Automated NEBNext^® Ultra™ II DNA Library Preparation for Illumina^®

Improved Library Preparation with NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®

Accelerating DNA Construction to Protein Expression: A Rapid 1-Day Workflow Using NEBridge^® Golden Gate Assembly

NEBNext^® Enzymatic Methyl-seq (EM-seq™)

Multiplex real-time PCR detection of monkeypox virus using Luna^® qPCR Reagents

Automated Directional RNA-seq Library Preparation using the Biomek FX^P Liquid Handler

Automated library preparation using NEBNext^® Ultra™ II Directional RNA Library Prep Kit for Illumina^® on the epMotion^® 5075t

Obtain superior NGS library performance with lower input amounts using the NEBNext^® Ultra™ II Directional RNA Library Prep Kit for Illumina^®

Improving NGS library performance with lower input amounts using the NEBNext^® Ultra™ II RNA Library Prep Kit for Illumina (non-directional)

Fully Automated NEBNext^® Direct Genotyping Solution using Beckman Coulter^® Life Sciences Biomek i7 Workstation

Biomek i-Series Automated New England Biolabs NEBNext^® Ultra™ II DNA Library Prep Kit

Increased transcription detection with the NEBNext^® Single Cell/Low Input RNA Library Prep Kit

Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch^® Plasmid Miniprep Kit

Determining Efficiency of On-Target CRISPR Cas9 Genome Editing Using the NEB^® EnGen^® Mutation Detection Kit on LabChip Gel Xpress

DNA Shuffling using NEBridge^® Golden Gate Assembly for Protein Engineering

Nanoliter Scale DNA Assembly Utilizing the NEBuilder^® HiFi Cloning Kit with the Labcyte^® Echo^® 525 Liquid Handler

Robust Colony PCR from Multiple E. coli Strains using OneTaq^® Quick-Load^® Master Mixes

Improved methods for site-directed mutagenesis using Gibson Assembly^® Master Mix

Using the PURExpress^® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants

Multiplex PCR using Q5^® High-Fidelity DNA Polymerase

Transformation of SHuffle^® Competent Cell Strains

Labeling of Escherichia coli Expressed SNAP-tag^® Fusion Proteins

Optimizing rapid isothermal workflow for SARS-CoV-2 with WarmStart^® LAMP with UDG

Improved methods for site-directed mutagenesis using NEBuilder^® HiFi DNA Assembly Master Mix

SNAP_f -based pulse labeling for analysis of protein turnover in living cells

NEBExpress^®Cell-free E. coli Protein Synthesis System

Optimized Integration of New England Biolabs^® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments

Improved method for assembly of linear yeast expression cassettes using NEBuilder^® HiFi DNA Assembly Master Mix