Home Protocols Typical GluC Digest Reaction Conditions (P8100)

Typical GluC Digest Reaction Conditions (P8100)

Endoproteinase GluC digestions are typically set up with a specific ratio of substrate protein to protease in the range of (20-50) : (1)

  1. Reconstitute Endoproteinase GluC in 500 µl H2O
  2. Combine:
    4 µl H2O
    5 µl protein substrate (1 µg)
    10 µl 2X GluC Reaction Buffer
    0.5 µl 100 ng/µl Endoproteinase GluC
  3. Incubate at 37°C for 16 hrs