Typical Reaction Conditions for Endoproteinase LysC (P8109)

For difficult to digest proteins or general proteomics applications, substrate may be reduced, denatured and alkylated prior to LysC digest.
  1. Combine 10-100 ng/μl substrate protein, 10 mM DTT, 30 mM iodoacetic acid and 5-8 M urea.
  2. Incubate in the dark 30 minutes.
  3. Add LysC; 1:20–1:100 LysC : Substrate ratio by mass.
  4. Incubate reaction at 37°C for 2-18 hours.