Cell Imaging
Choose Type:
- Cellular Labeling (E9100)
- Cellular Labeling (S9103)
- Cellular Labeling (S9104)
- Cellular Labeling (S9105)
- Cellular Labeling (S9107)
- Cellular Labeling (S9109)
- Cellular Labeling (S9110)
- Cellular Labeling (S9112)
- Cellular Labeling (S9124)
- Cellular Labeling (S9126)
- Cellular Labeling (S9129)
- Cellular Labeling (S9131)
- Cellular Labeling (S9132)
- Cellular Labeling (S9134)
- Cellular Labeling (S9136)
- Cellular Labeling (S9137)
- Cellular Labeling (S9139)
- Cellular Labeling (S9142)
- Cellular Labeling (S9216)
- Cellular Labeling (S9217)
- Cellular Labeling (S9218)
- Cellular Labeling (S9219)
- Cellular Labeling (S9232)
- Cellular Labeling (S9233)
- Cellular Labeling (S9234)
- Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
- Cloning of CLIP-tag Fusions in pCLIPf (N9215)
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- CoA 488 (S9348)
- Expression of ACP-ADRβ2 Fusions (N9321)
- Expression of ACP-tag Fusions (N9322)
- Expression of CLIP-tag Fusions (N9215)
- Expression of MCP Fusions (N9317)
- Expression of MCP-GPI (N9320)
- Cellular Labeling (S9101)
- Instructions for Cellular Labeling (S9215)
- Instructions for Use with ACP Synthase (P9301)
- Instructions for Use with SFP Synthase (P9302)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9236)
- Labeling Mammalian Cell Lysates (S9147)
- Labeling of Proteins in vitro (S9110)
- Labeling of Proteins in vitro (P9302)
- Labeling of Proteins in vitro (S9103)
- Labeling of Proteins in vitro (S9104)
- Labeling of Proteins in vitro (S9105)
- Labeling of Proteins in vitro (S9106)
- Labeling of Proteins in vitro (S9107)
- Labeling of Proteins in vitro (S9109)
- Labeling of Proteins in vitro (S9137)
- Labeling of Proteins in vitro (S9143)
- Labeling of Proteins in vitro (S9216)
- Labeling of Proteins in vitro (S9217)
- Labeling of Proteins in vitro (S9218)
- Labeling of Proteins in vitro (S9219)
- Labeling of Proteins in vitro (S9220)
- Labeling of Proteins in vitro (S9221)
- Labeling of Proteins in vitro (S9232)
- Labeling of Proteins in vitro (S9233)
- Labeling of Proteins in vitro (S9234)
- Labeling of Proteins in vitro (P9301)
- Instructions for Labeling of Proteins in vitro (S9348)
- Labeling of Proteins in vitro (S9101)
- Labeling of Proteins in vitro (S9351)
- Labeling on the Surface of Cells (S9349)
- Labeling on the Surface of Cells (S9350)
- Labeling on the Surface of Cells (S9351)
- Labeling Proteins in vitro (S9131)
- Labeling Proteins in vitro (S9112)
- Labeling Proteins in vitro (S9124)
- Labeling proteins in vitro (S9126)
- Labeling Proteins in vitro (S9129)
- Labeling Proteins in vitro (S9132)
- Labeling Proteins in vitro (S9134)
- Labeling Proteins in vitro (S9136)
- Labeling Proteins in vitro (S9139)
- Labeling Proteins in vitro (S9142)
- Labeling Proteins in vitro (S9147)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9150)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9151)
- Labeling SNAP-tag Purified Protein In Vitro (P9312)
- Protocol for Anti-SNAP-tag® Antibody (Polyclonal) (P9310)
- Use of SNAP-Cell Block with SNAP-Cell Substrates (E9100)
- Use with CLIP-tag substrates (S9220)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Instructions for Cellular Labeling (E9200)
- Labeling Proteins in vitro (E9200)
- Labeling of Proteins in vitro (S9215)
- Cloning of SNAP-tag Fusions in pSNAPf (N9183)
- Cloning of SNAP-tag Fusions in pSNAP-tag(T7)-2 (N9181)
- Expression of SNAP-tag Fusions (N9181)
- Expression of SNAPf Fusions (N9183)
- Expression of SNAPf Fusions (N9185)
- Expression of SNAPf Fusions in SNAPf-H2B (N9186)
- Labeling of Proteins in vitro (S9349)
- Labeling of Proteins in vitro (S9350)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9148)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)
- Cellular Labeling (S9221)
- Labeling of Proteins in vitro (E9100)
- Use with SNAP-Surface substrates (S9143)
- Reaction Conditions for Chemical Coupling (S9148)
- Reaction Conditions for Chemical Coupling (S9153)
- Use SNAP-Capture Magnetic Beads (S9145)
- Use SNAP-Capture Pull Down Resin (S9144)
- Expression of CLIPf-Cox8A (N9217)
- Expression of CLIPf-H2B (N9218)
- Reaction Conditions for Chemical coupling (S9150)
- Reaction Conditions for Chemical Coupling (S9151)
- Reaction Conditions for Chemical Coupling (S9236)
- Use with SNAP-Cell Substrates (S9106)
-
SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
- Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A (2019) A ligand based system for receptor specific delivery of proteins Sci Rep; 9(1), 19214.. PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
- Reuter, W.H., Masuch, T., Ke, N., Lenon, M., Radzinski, M., Van Loi, V., Ren, G., Riggs, P., Antelmann, H., Reichmann, D., Leichert, L.I., Berkmen, M (2019) Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote Redox Biol; 26, 101280. PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
- Carpinone, E.M., Li, Z., Mills, M.K., Foltz, C., Brannon, E.R., Carlow, C.K.S., Starai, V.J. (2018) Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi PLoS One; 13 (9), e0204736. PubMedID: 30261054, DOI: 10.1371/journal.pone.0204736
- Ke, N., Landgraf, D., Paulsson, J. and Berkmen, M. (2016) Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag. J Bacteriol; Jan 19;198(7), 1035-43. PubMedID: 26787765
- Clone and express once, then use with a variety of substrates
- Non-toxic to living cells
- Wide selection of fluorescent substrates
- Highly specific covalent labeling
- Simultaneous dual labeling
- Simultaneous dual protein labeling inside live cells
- Protein localization and translocation
- Pulse-chase experiments
- Receptor internalization studies
- Selective cell surface labeling
- Protein pull-down assays
- Protein detection in SDS-PAGE
- Flow cytometry
- High throughput binding assays in microtiter plates
- Biosensor interaction experiments
- FRET-based binding assays
- Single molecule labeling
- Super-resolution microscopy
- Small - Expressed tag is only 8 kDa (77aa)
- Versatile - ACP- and MCP-tagged fusions can be co-expressed and sequentially labeled for two color applications on cell surfaces
- Specific - Components remain exclusively extracellular, preventing intracellular labeling
- Precise - Label is covalently bound under biological conditions in a defined position
- Non-toxic - Substrates are non-toxic to living cells
- Selection - Choice of substrates available, including 488, 547, 647 nm and biotin
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.