Thermostable FEN1

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0645S     -20       Thermostable FEN1 M0645SVIAL -20 1 x 0.05 ml 32,000 units/ml   ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X

Application Features

• Cleavage of flap DNA structure

Materials Sold Separately

• ThermoPol® Reaction Buffer Pack

1. Balakrishnon, L. et al. (2013). Annu. Rev. Biochem. 82, 119-138.
2. Greenough, L. et al. (2015). J. Biol. Chem. 20, 12514-12522.
3. Greenough, L. et al. (2015). Nucleic Acids Res. DOI: doi:10.1093/nar/gkv899 PubMedID: 26365239

1. Thermostable FEN1 Reaction Protocol for Cleavage of Flap DNA Structure (NEB #M0645)

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

1. What is the activity of Thermostable FEN1 in various NEB Buffers?
2. What is the activity of Thermostable FEN1 at various temperatures?
3. What is the preferred substrate for Thermostable FEN1?
4. What is the largest 5´ DNA flap Thermostable FEN1 will cleave?
5. What is the smallest 5´ DNA flap Thermostable FEN1 will cleave?
6. What are the various substrates that Thermostable FEN1 activity has been tested on, and what is the % Activity on each substrates?
7. Can I purchase large amounts of an existing Enzyme for Innovation?
8. What are Enzymes for Innovation?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0645S_L_B_v1
• M0645S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0645S_v1_10012370
• M0645S_v1_10021348
• M0645S_v1_10043261
• M0645S_v1_10050210
• M0645S_v1_10059586
• M0645S_v1_10072358
• M0645S_v1_10078894
• M0645S_v1_10088840
• M0645S_v1_10098975
• M0645S_v1_10150951
• M0645S_v1_10178531
• M0645S_v1_10197834
• M0645S_v1_10215703
• M0645S_v1_10222850
• M0645S_v1_10247074
• M0645S_v1_10262041
• M0645S_v1_10268438
• M0645S_v1_10275808
• M0645S_v1_10286945
• M0645S_v1_10303773
• M0645S_v1_10314260
• M0645S_v1_10320062
• M0645S_v1_10323053

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Thermostable FEN1
• ThermoPol^® Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.