NEBNext® LV Unique Dual Index Primers Set 2B
Please note: Beginning 06/01/2026 and phasing in on a rolling basis, NEBNext Multiplex Oligos provided in a 96-well plate will be updated to Eppendorf twin.tec® PCR Plates (Eppendorf #951020401) with Agilent Pierceable Aluminum Seals (Agilent #06644-001). This product has not yet been updated. Please contact NEBNext Technical Support (info@neb.com) with any questions related to these updates.
NEBNext® LV Unique Dual Index Primers Set 2B is the new name for NEBNext Primers for Epigenetics (Unique Dual Index Set 2B); no other changes have been made to the product.
NEBNext LV Unique Dual Index Primers Set 2B includes 24 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ v2, E5hmC-seq™, and NEBNext Low-bias Small RNA Library Prep Kit, with Illumina® sequencing. The primers enable incorporation of unique index pairs during library amplification and have been designed to minimize index hopping.
- Optimized for low-volume (LV) NEBNext library prep workflows
- Addresses the "barcode hopping" issue seen with some Illumina sequencing instruments
- Increases sample identification specificity with unique dual index primer pairs
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Product Information
NEBNext LV Unique Dual Index Primers have been selected and paired for optimal multiplex index incorporation during PCR. Optimized for low-volume (LV) NEBNext workflows, including EM-seq v2 and E5hmC-seq, these primers enable multiplexing and have been designed to minimize index hopping. They are supplied as pairs of premixed i5 and i7 8-base index primers in 24 wells of a plate that has a pierceable foil seal for easy, single use.
The index sequences from the NEBNext LV Unique Dual Index Primers Set 2B are compatible with and can be combined with the other NEBNext LV Unique Dual Index Primers sets to multiplex up to 480 reactions. Note: NEBNext LV Unique Dual Index Primers Set 2A (NEB #E3390) and Set 2B are 24-index subsets of Set 2 (NEB #E3402); therefore, we do not recommend combining Set 2A and/or 2B with Set 2
Please refer to the relevant NEBNext library prep kit manual for usage instructions. Sample sheets for use with these NEBNext LV Unique Dual Index Primers can be found under "Usage Guidelines" on this product page (see below).
- This product is related to the following categories:
- Hydroxymethylation Detection and Analysis,
- Enzymatic Conversion for DNA Methylation Analysis,
- Methylome Analysis,
- NEBNext® Multiplex Oligos (Adaptors & Primers),
- DNA Methylation Analysis, Next Generation Sequencing Library Preparation
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