Home PCR, qPCR & Amplification Technologies Bst DNA Polymerase, Full Length

Bst DNA Polymerase, Full Length
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The L size of this product has been discontinued.  The S size will still be available.

Optimal performance in a number of applications

  • Isothermal DNA amplification
  • Primer extension
  • Utilizes ThermoPol® Reaction Buffer for optimum reaction conditions

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Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
M0328S 5,000 units/ml 500 units $87.00 $87.00
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  • Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity (1).

    Product Source

    An E. coli strain that contains the gene from Bacillus stearothermophilus. (H. Kong)
    This product is related to the following categories:
    Isothermal Amplification & Strand Displacement
    This product can be used in the following applications:
    Isothermal Amplification

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

      Reaction Conditions

      1X ThermoPol® Reaction Buffer
      Incubate at 65°C

      1X ThermoPol® Reaction Buffer
      20 mM Tris-HCl
      10 mM (NH4)2SO4
      10 mM KCl
      2 mM MgSO4
      0.1% Triton® X-100
      (pH 8.8 @ 25°C)

      Storage Buffer

      10 mM Tris-HCl
      50 mM KCl
      1 mM DTT
      0.1 mM EDTA
      50% Glycerol
      0.1% Triton® X-100
      pH 7.1 @ 25°C

      Heat Inactivation

      80°C for 20 minutes

      5' - 3' Exonuclease

      Yes

      3' - 5' Exonuclease

      No

      Strand Displacement

      No

      Unit Assay Conditions

      50 mM KCl, 20 mM Tris-HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24-mer, 200 µM dATP, 200 µM dCTP, 200 µM dGTP, 100 µM dTTP including [3H]-dTTP and 100 µg/ml BSA.

    • Related Products

      Companion Products

      Materials Sold Separately

    • Product Notes

      1. Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
      2. Reaction temperatures above 70°C are not recommended.
      3. Cannot be used for thermal cycle sequencing or PCR.

    • References

      1. Aliotta, J.M. et al. (1996). Genetic Analysis. 12, 185-195.
      2. Mead, D.A. et al. (1991). BioTechniques. 11, 76-87.

  • Protocols, Manuals & Usage

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      Effective March 15, 2022, RNase method incubation time has changed to 4 hours.
      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

      Trademarks

      NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
      TRITON® is a registered trademark of Union Carbide Corporation.

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