DNA Amplification

Fast PCR

The speed of a PCR can depend on a number of factors, including the extension rate of the polymerase used, the ramp speed of the thermocycler and the complexity of the DNA template. Compared to standard extension rates of Taq DNA Polymerase (~1kb/min), enzymes that are able to perform fast PCR have extension rates that are typically 2-4kb/min.

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Protocols for Fast PCR
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Fast PCR
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Deoxynucleotide (dNTP) Solution Mix

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Deoxynucleotide (dNTP) Solution Set

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NEBNext® High-Fidelity 2X PCR Master Mix

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NEBNext® Q5® Hot Start HiFi PCR Master Mix

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Phusion GC Buffer Pack

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Phusion HF Buffer Pack

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Phusion High-Fidelity DNA Polymerase

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Phusion High-Fidelity PCR Master Mix with GC Buffer

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Phusion High-Fidelity PCR Master Mix with HF Buffer

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Phusion Hot Start Flex 2X Master Mix

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Phusion Hot Start Flex DNA Polymerase

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Phusion High-Fidelity PCR Kit

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Q5® High-Fidelity 2X Master Mix

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Q5® High-Fidelity DNA Polymerase

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Q5® High-Fidelity PCR Kit

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Q5® Hot Start High-Fidelity 2X Master Mix

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Q5® Hot Start High-Fidelity DNA Polymerase

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Q5® Reaction Buffer Pack

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Q5®-XT Hot Start High-Fidelity 2X Master Mix

Videos

  • Types of PCR

    High-fidelity, multiplex, and Fast PCR are just a few of the types of amplification reactions you may need to conduct. NEB offers a variety of DNA polymerases for all of your experimental needs. Breton explains which polymerases to choose.