OneTaq® 2X Master Mix with Standard Buffer
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT / GC content
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
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Product Information
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Figure 1: Comparison of OneTaq products to other commercially available polymerases
Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity. ![[Icon] Use our Tm Calculator to determine your primers' optimal annealing temperature.](https://media.neb.com/asset/46e6c17d-deda-4972-a20c-2c3463d03d8b/Webp_JPG_PSD/TmCalculator_Banner.webp?=)
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.- This product is related to the following categories:
- OneTaq® DNA Polymerases,
- Master Mixes,
- Taq DNA Polymerase
- This product can be used in the following applications:
- Fast PCR,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR, PCR
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Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Citations & Technical Literature
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Additional Citations
- Stöhr AC, López-Bueno A, Blahak S, Caeiro MF, Rosa GM, Alves de Matos AP, Martel A, Alejo A, Marschang RE (2015) Phylogeny and differentiation of reptilian and amphibian ranaviruses detected in europe PLoS One; 10(2), e0118633. PubMedID: 25706285, DOI: 10.1371/journal.pone.0118633
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