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NEBNext® Sample Preparation for Next Generation Sequencing

Preparation of high quality libraries at high yield is a critical first step in next generation sequencing workflows. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA and RNA and target enrichment of DNA for next generation sequencing. NEBNext kits are carefully designed to improve yields and library diversity using a broad range of input amounts from a wide variety of sample types. This has been most recently expanded with the addition of a novel enzyme-based DNA fragmentation system in the NEBNext Ultra II FS DNA Library Prep Kit (NEB #E7805), which enables reliable high-yield high-quality library construction with as little as 100 pg of input DNA.

Another recent technology addition to the NEBNext product line is NEBNext Direct® for target enrichment. This unique hybridization-based technology enables high sensitivity variant calling with the speed and precision previously only feasible using multiplex PCR-based approaches. The NEBNext Direct Cancer HotSpot Panel (NEB #E7000) provides highly specific hybridization-based capture of 190 common cancer targets from 50 genes, while the NEBNext Direct BRCA1/BRCA2 Panel (NEB #E6627) generates 100% coverage of all protein coding regions in BRCA1 and BRCA2 genes.


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NEBNext® Sample Preparation for Next Generation Sequencing includes these areas of focus:

Illumina® Library Preparation
RNA for Illumina®
DNA for Illumina®
NEBNext® Ultra™ II DNA Library Prep
NEBNext® Ultra™ II RNA Library Prep
Ion Torrent™ DNA Library Preparation
DNA for Ion Torrent™
454™ Library Preparation
DNA for 454™
RNA for 454™
DNA Library Preparation
RNA Library Preparation
DNA Fragmentation
FFPE DNA Repair
NEBNext FFPE DNA Repair Mix
NEBNext® Automation

FAQs for NEBNext® Sample Preparation for Next Generation Sequencing

Protocols for NEBNext® Sample Preparation for Next Generation Sequencing

    Publications related to NEBNext® Sample Preparation for Next Generation Sequencing

  1. Luck, A.N., Anderson, K.G., McClung, C.M., VerBerkmoes, N.C., Foster, J.M., Michalski, M.L. and Slatko, B.E. 2015. Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont BMC Genomics. , PubMedID: 26559510, DOI:

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. NEBNextUltra2DirectionalRNA_720

    NEBNext Ultra II Directional RNA Workflow

    Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.

  2. Size Selection and Cleanup with NEBNext Ultra

    Size Selection and Cleanup with NEBNext Ultra II and SPRI beads

    This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA

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    Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

  4. NEBNextVideo_OptimizingRNAInput_thumb

    Tips for Optimizing RNA Inputs in NGS Library Construction

    Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.

  5. NEBNext Direct Workflow

    NEBNext Direct® Workflow

    View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow.

  6. NEBNext Direct Target Enrichment

    NEBNext Direct® Target Enrichment

    Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.

  7. NEBTV Webinar

    How Library Preparation Affects Sequencing Accuracy

    In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary.

  8. Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification

    Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification

    NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.

  9. NEBNext_Direct_Webinar_282x210

    Challenges and Opportunities for Next Generation Sequencing Target Enrichment

    Target enrichment offers several advantages over more comprehensive genomic profiling for a variety of scientific and applications. During this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct®, a novel solution for selective enrichment of genomic targets, addresses these challenges.

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    NEBNext Ultra II DNA Library Prep Protocol

    This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

  11. NEBNextVideo_CoreLabFacilities_thumb

    NEBNext for Core Lab Facilities

    Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.

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    12 Quick Tips for NGS Library Preparation

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.