Next Generation Sequencing

NEBNext® Sample Preparation for Next Generation Sequencing

Preparation of high quality libraries at high yield is a critical first step in next generation sequencing workflows. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA and RNA and target enrichment of DNA for next generation sequencing. NEBNext kits are carefully designed to improve yields and library diversity using a broad range of input amounts from a wide variety of sample types. For example, each reagent in the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) has been reformulated, resulting in a several-fold increase in library yield with as little as 500 pg of input DNA.

A recent technology addition to the NEBNext product line is NEBNext Direct™ for target enrichment. This unique hybridization-based technology enables high sensitivity variant calling with the speed and precision previously only feasible using multiplex PCR-based approaches. The NEBNext Direct Cancer HotSpot Panel provides highly specific hybridization-based capture of 190 common cancer targets from 50 genes.

Use the following tool to determine the best NEBNext products for you:

NEBNext® is a registered trademark and NEBNext Direct™ is a trademark of New England Biolabs, Inc.




  1. NEBNext Direct® Target Enrichment

    Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.

  2. NEBNext Ultra II DNA Library Prep Protocol

    This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

  3. 12 Quick Tips for NGS Library Preparation

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

  4. Tips for Optimizing RNA Inputs in NGS Library Construction

    Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.

  5. Which NEBNext Product Format Should I Choose?

    Not sure how sets and kits differ from modules? Ping explains the difference, and helps you to decide which one is right for you.

  6. NEBNext for Core Lab Facilities

    Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.

  7. Which DNA Library Prep Kit for Ion Torrent Should I Choose?

    Having trouble deciding which NEBNext Fast DNA Library Prep Set for Ion Torrent™ to choose? Let Christine explain which set is right for your DNA.

  8. Tips for Bead-based Clean ups and Size Selection

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

  9. Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

  10. Optimization of NGS Library Preparation: Low Inputs and Fast, Streamlined Workflows

    Learn how NEBNext® Ultra™ is enabling library prep for multiple applications, with lower input amounts and fast, streamlined workflows. In this GenomeWeb webinar Cynthia Hendrickson from HudsonAlpha Institute of Biotechnology, Momo Vuyisich from Los Alamos National Laboratory and Daniela Munafo from New England Biolabs describe use of NEBNext Ultra in exome sequencing, bacterial genome resequencing and assembly, and strand-specific RNA-Seq.

  11. A Breakthrough Method of Small RNA Sample Preparation

    The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

NEBNext® Sample Preparation for Next Generation Sequencing includes these areas of focus:

Illumina® Library Preparation
NEBNext® Ultra™ II DNA Library Prep
NEBNext® Ultra™ II RNA Library Prep
DNA for Illumina®
RNA for Illumina®
Ion Torrent™ DNA Library Preparation
DNA for Ion Torrent™
454™ Library Preparation
DNA for 454™
RNA for 454™
SOLiD™ Library Preparation
DNA for SOLiD™
RNA for SOLiD™
DNA Library Preparation
RNA Library Preparation
DNA Fragmentation
NEBNext® dsDNA Fragmentase®
FFPE DNA Repair
NEBNext FFPE DNA Repair Mix
NEBNext® Automation

FAQs for NEBNext® Sample Preparation for Next Generation Sequencing

Protocols for NEBNext® Sample Preparation for Next Generation Sequencing

    Publications related to NEBNext® Sample Preparation for Next Generation Sequencing:

  1. Luck, A.N., Anderson, K.G., McClung, C.M., VerBerkmoes, N.C., Foster, J.M., Michalski, M.L. and Slatko, B.E. (2015). Tissue-specific transcriptomics and proteomics of a filarial nematode and its Wolbachia endosymbiont BMC Genomics. 12864-015-2083-2. PubMedID: 26559510

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.