NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible kit enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.
NEBuilder HiFi DNA assembly offers unique features over other DNA assembly methods, including:
- 3´- and 5´-end mismatch removal
- Bridging of two ds-fragments with a ssDNA oligo
- Improved methods for site-directed mutagenesis
View performance data against NEB Gibson Assembly® and In-Fusion® HD.
To help select the best DNA assembly method for your needs, please use our Synthetic Biology/DNA Assembly Selection Chart.