NEBuilder® HiFi DNA Assembly

Assembling DNA fragments is a key part of both synthetic biology techniques and cloning. NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible mix enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.

5 Reasons to choose NEBuilder HiFi 

  1. Save time – Enjoy simple and fast seamless cloning in as little as 15 minutes.
  2. Flexibility – Use one system for both "standard-size" cloning and larger gene assembly products, up to 12 fragments.
  3. Compatible with downstream applications – DNA can be used immediately for transformation or as template for PCR or RCA.
  4. Adaptable – Adapts easily for multiple DNA manipulations, including site-directed mutagenesis. 
  5. Save money - No licensing fees from NEB for NEBuilder products

Want to try NEBuilder HiFi DNA Assembly?

 

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NEBuilder HiFi DNA assembly offers unique features over other DNA assembly methods, including:

 
How does NEBuilder HiFi stack up?

graph iconPerformance data and advantages against GeneArt® Gibson Assembly® and In-Fusion® Snap Assembly 
table iconSynthetic Biology/DNA Assembly Selection Chart
table iconComparison of DNA Assembly Reaction Types
table iconGuidelines for using NEBuilder HiFi DNA Assembly
table iconHigh-throughput cloning and automation solutions

 
Not your average DNA assembly reagent

NEBuilder HiFi DNA Assembly can be used for a variety of DNA assembly methods.

 

 
NEBuilder HiFi products

NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520)
  • Includes NEB 5-alpha competent cells
  • NEB recommends NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) for routine assemblies of 15 kb or less
  • For repetitive sequences, NEB recommends NEB Stable Competent E. coli (NEB #C3040)
NEBuilder HiFi DNA Assembly Bundle for Large Fragments (NEB #E2623)
  • Includes NEB 10-beta competent cells
  • NEB recommends NEB 10-beta Competent E. coli (High Efficiency, NEB #C3019) or NEB 10-beta Electrocompetent E. coli (NEB #C3020) for assemblies larger than 15 kb
NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621)
  • Does not include competent cells 

 

Online Supporting Tools

NEBuilder_WebTool_NEB NEBuilder® Assembly Tool
Use to easily design primers for your NEBuilder HiFi DNA Assembly reactions. 
NEBuilder® Protocol Calculator
NEBuilder® Protocol Calculator
Use to generate a customized protocol for your NEBuilder HiFi DNA Assembly reaction.
NEBaseChanger_WebTool_NEB
NEBaseChanger® 
For multi-site mutagenesis primer design using NEBuilder HiFi DNA Assembly.

 

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at busdev@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications. 


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FAQs for NEBuilder® HiFi DNA Assembly
Protocols for NEBuilder® HiFi DNA Assembly
Application Notes for NEBuilder® HiFi DNA Assembly
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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