NEBuilder^® HiFi DNA Assembly

Assembling DNA fragments is a key part of both synthetic biology techniques and cloning. NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible mix enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.

5 Reasons to choose NEBuilder HiFi 

1. Save time – Enjoy simple and fast seamless cloning in as little as 15 minutes.
2. Flexibility – Use one system for both "standard-size" cloning and larger gene assembly products, up to 11 fragments.
3. Compatible with downstream applications – DNA can be used immediately for transformation or as template for PCR or RCA.
4. Adaptable – Adapts easily for multiple DNA manipulations, including site-directed mutagenesis. 
5. Save money - No licensing fees from NEB for NEBuilder products

Want to try NEBuilder HiFi DNA Assembly?

 

Request a Sample 

NEBuilder HiFi DNA assembly offers unique features over other DNA assembly methods, including:

• 3´- and 5´-end mismatch removal 
• Bridging of two ds-fragments with a ssDNA oligo
• Improved methods for site-directed mutagenesis

How does NEBuilder HiFi stack up?

Performance data and advantages against GeneArt^® Gibson Assembly^® and In-Fusion^® Snap Assembly 
Synthetic Biology/DNA Assembly Selection Chart
Comparison of DNA Assembly Reaction Types
Guidelines for using NEBuilder HiFi DNA Assembly

Not your average DNA assembly reagent

NEBuilder HiFi DNA Assembly can be used for a variety of DNA assembly methods.

NEBuilder HiFi products

• Includes NEB 5-alpha competent cells
• NEB recommends NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) for routine assemblies of 15 kb or less
• For repetitive sequences, NEB recommends NEB Stable Competent E. coli (NEB #C3040)

• Includes NEB 10-beta competent cells
• NEB recommends NEB 10-beta Competent E. coli (High Efficiency, NEB #C3019) or NEB 10-beta Electrocompetent E. coli (NEB #C3020) for assemblies larger than 15 kb

• Does not include competent cells 

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at busdev@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.