Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.

Diluent Buffer A
Acc65I AccI AciI AflII AgeI-HF®
AhdI AlwI AlwNI ApaI ApaLI
ApoI§ AscI AvaI AvaII BaeGI
BaeI BamHI§ BamHI-HF® BanI BanII
BccI BceAI BcgI BclI BglII
BlpI BsaJI BsaWI BseRI BsiEI
BsiHKAI BslI BsmFI BsmI BsoBI
Bsp1286I BspCNI BspDI BspHI BsrBI
BsrDI BsrGI§ BsrGI-HF® BstAPI BstBI
BstEII § BstEII-HF® BstNI BstUI BstYI
BstZ17I-HF® BtgZI BtsαI BtsIMutI ClaI
CspCI CviKI-1 DraI DrdI EaeI
EciI Eco53kI EcoNI EcoO109I EcoP15I
EcoRV§ FatI FauI Fnu4HI FokI
HaeII HaeIII HgaI HhaI HinfI
HinP1I HpaI HpaII Hpy188I Hpy99I
HpyCH4III HpyCH4IV HpyCH4V KpnI§ KpnI-HF®
MboI MfeI§ MfeI-HF® MluCI MluI§
MluI-HF® MlyI MseI MslI MspI
NaeI NarI Nb.BbvCI Nb.BsmI Nb.BsrDI
Nb.BtsI NciI NcoI§ NdeI NgoMIV
NotI-HF® NruI§ NruI-HF® NspI Nt.AlwI
Nt.BbvCI Nt.BsmAI Nt.BstNBI PacI PaeR7I
PflFI PflMI PleI PluTI PmeI
PmlI PshAI PspGI RsaI SacI§
SacI-HF® SacII SalI§ SalI-HF® Sau3AI
Sau96I SbfI§ SexAI SgrAI SmlI
SnaBI StuI StyI§ StyI-HF® Tsp45I
XbaI XhoI XmaI XmnI
Diluent Buffer B
AatII AclI AcuI AfeI AflIII
AleI AluI ApeKI ApoI-HF AseI
AsiSI AvrII BbsI§ BbsI-HF® BbvCI
BbvI BclI-HF BcoDI BfaI BfuAI
BfuCI BglI BmgBI BmrI BmtI§
BmtI-HF® BpmI Bpu10I BpuEI BsaBI
BsaI§ BsaI-HF® BseYI BsgI BsiWI§
BsiWI-HF® BsmAI BsmBI BspEI BspMI
BspQI BsrI BssHII BssSαI BstXI
BtgI BtsCI Cac8I DdeI DpnI
DpnII DraIII-HF® EagI§ EagI-HF® EarI
EcoRV-HF® FseI FspEI HincII HindIII§
HindIII-HF® HphI Hpy188III I-CeuI I-SceI
KasI LpnPI MmeI MnlI MspA1I
MspJI MwoI Nb.BssSI NcoI-HF® NlaIII
NlaIV NmeAIII NsiI§ NsiI-HF® Nt.BspQI
PciI PI-PspI PI-SceI PpuMI PsiI
PspOMI PspXI PvuI§ PvuI-HF® PvuII§
PvuII-HF® SapI SbfI-HF® ScaI-HF® SfaNI
SfcI SfoI SmaI SphI§ SphI-HF®
SrfI SspI-HF® StyD4I SwaI TaqαI
TseI TspMI TspRI Tth111I ZraI
Diluent Buffer C
AbaSI AgeI§ BciVI BsaAI BsaHI
BsaXI BsrFαI Bsu36I CviAII CviQI
EcoRI§ EcoRI-HF® FspI Hpy166II MboII
MscI NheI§ NheI-HF® NotI§ PstI§
PstI-HF® RsrII ScrFI SfiI SpeI§
SpeI-HF® SspI§ TfiI XcmI

§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.
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