Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.

Diluent Buffer A
Acc65IAccIAciIAflIIAgeI-HF®
AhdIAlwIAlwNIApaIApaLI
ApoI §AscIAvaIAvaIIBaeGI
BaeIBamHI §BamHI-HF®BanIBanII
BccIBceAIBcgIBclIBglII
BlpIBsaJIBsaWIBseRIBsiEI
BsiHKAIBslIBsmFIBsmIBsoBI
Bsp1286IBspCNIBspDIBspHIBsrBI
BsrDIBsrGI §BsrGI-HF®BssKIBstAPI
BstBIBstEII  §BstEII-HF®BstNIBstUI
BstYIBstZ17I-HF®BtgZIBtsαIBtsI
BtsIMutIClaICspCICviKI-1DraI
DrdIEaeIEciIEco53kIEcoNI
EcoO109IEcoP15IEcoRV §FatIFauI
Fnu4HIFokIHaeIIHaeIIIHgaI
HhaIHinfIHinP1IHpaIHpaII
Hpy188IHpy99IHpyCH4IIIHpyCH4IVHpyCH4V
KpnI §KpnI-HF®MboIMfeI §MfeI-HF®
MluCIMluI §MluI-HF®MlyIMseI
MslIMspINaeINarINb.BbvCI
Nb.BsmINb.BsrDINb.BtsINciINcoI §
NdeINgoMIVNotI-HF®NruI §NruI-HF®
NspINt.AlwINt.BbvCINt.BsmAINt.BstNBI
Nt.CviPIIPacIPaeR7IPflFIPflMI
PhoIPleIPluTIPmeIPmlI
PshAIPspGIRsaISacI §SacI-HF®
SacIISalI §SalI-HF®Sau3AISau96I
SbfI §ScaI §SexAISgrAISmlI
SnaBIStuIStyI §StyI-HF®Tsp45I
Tsp509IXbaIXhoIXmaIXmnI
Diluent Buffer B
AatIIAclIAcuIAfeIAflIII
AleIAluIApeKIApoI-HFAseI
AsiSIAvrIIBbsI §BbsI-HF®BbvCI
BbvIBclI-HFBcoDIBfaIBfuAI
BfuCIBglIBmgBIBmrIBmtI §
BmtI-HF®BpmIBpu10IBpuEIBsaBI
BsaI §BsaI-HF®BseYIBsgIBsiWI §
BsiWI-HF®BsmAIBsmBIBspEIBspMI
BspQIBsrIBssHIIBssSαIBssSI
BstXIBstZ17I §BtgIBtsCICac8I
DdeIDpnIDpnIIDraIII §DraIII-HF®
EagI §EagI-HF®EarIEcoRV-HF®FseI
FspEIHincIIHindIII §HindIII-HF®HphI
Hpy188IIII-CeuII-SceIKasILpnPI
MmeIMnlIMspA1IMspJIMwoI
Nb.BssSINcoI-HF®NlaIIINlaIVNmeAIII
NsiI §NsiI-HF®Nt.BspQIPciIPI-PspI
PI-SceIPpuMIPsiIPspOMIPspXI
PvuI §PvuI-HF®PvuII §PvuII-HF®SapI
SbfI-HF®ScaI-HF®SfaNISfcISfoI
SmaISphI §SphI-HF®SrfISspI-HF®
StyD4ISwaITaqαITliITseI
TspMITspRITth111IZraI
Diluent Buffer C
AbaSIAgeI §BciVIBsaAIBsaHI
BsaXIBsrFαIBsrFIBsu36ICviAII
CviQIEcoRI §EcoRI-HF®FspIHpy166II
MboIIMscINheI §NheI-HF®NotI §
PstI §PstI-HF®RsrIIScrFISfiI
SpeI §SpeI-HF®SspI §TfiIXcmI
§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.