• My NEB
  • Print
  • PDF
  • Restriction Enzyme Diluent Buffer Compatibility

    Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

    Note: These diluents are not for use with Thermophilic DNA Polymerases. Thermophilic DNA Polymerases should be diluted with specialized diluents.

    Diluent Buffer A
    Acc65IAccIAciIAflIIAgeI-HF® (2)
    AhdIAlwIAlwNIApaIApaLI
    ApoIAscI (2)AvaIAvaIIBaeGI
    BaeIBamHI (1)BamHI-HF®BanIBanII
    BccIBceAIBcgIBclIBglII
    BlpIBsaHIBsaJIBsaWIBseRI
    BsiEIBslIBsmFIBsmIBsoBI
    Bsp1286IBspCNIBspDIBspHIBsrBI
    BsrDIBsrGI (1)BsrGI-HF®BssKIBstAPI
    BstBIBstEII  (1) (2)BstEII-HF®BstNIBstUI
    BstYIBsu36IBtgZIBtsαIBtsI
    BtsIMutIClaICspCICviKI-1DraI
    DrdIEaeIEciIEco53kIEcoNI
    EcoO109IEcoP15IEcoRV (1) (2)FatIFauI
    Fnu4HIFokIHaeIIHaeIIIHgaI
    HhaIHinfIHinP1IHpaIHpaII
    Hpy188IHpy99IHpyCH4IIIHpyCH4IVHpyCH4V
    KpnI (1) (2)KpnI-HF® (2)MboIMfeI (1) (2)MfeI-HF® (2)
    MluCIMluI (1)MluI-HF®MlyIMseI
    MslIMspINaeINarINb.BbvCI
    Nb.BsmINb.BsrDINb.BtsINciINcoI (1) (2)
    NdeINgoMIVNotI-HF® (2)NruI (1)NruI-HF®
    NspINt.AlwINt.BbvCINt.BsmAINt.BstNBI
    Nt.CviPIIPacI (2)PaeR7IPflFIPflMI
    PhoIPleIPluTIPmeIPmlI
    PshAIPspGIRsaISacI (1)SacI-HF®
    SacIISalI (1) (2)SalI-HF® (2)Sau3AISau96I
    SbfI (1)ScaI (1) (2)SexAISgrAISmlI
    SnaBIStuIStyI (1)StyI-HF®Tsp45I
    Tsp509IXbaI (2)XhoI (2)XmaIXmnI
    Diluent Buffer B
    AatIIAclIAcuIAfeIAflIII
    AleIAluIApeKIAseIAsiSI
    AvrIIBbsIBbvCIBbvIBcoDI
    BfaIBfuAIBfuCIBglIBmgBI
    BmrIBmtI (1)BmtI-HF®BpmIBpu10I
    BpuEIBsaBIBsaI (1)BsaI-HF®BsaXI
    BseYIBsgIBsiHKAIBsiWIBsmAI
    BsmBIBspEIBspMIBspQIBsrI
    BssHIIBssSαIBssSIBstXIBstZ17I
    BtgIBtsCICac8IDdeIDpnI
    DpnIIDraIII (1)DraIII-HF®EagI-HF®EarI
    EcoRV-HF® (2)FseIFspEIHincIIHindIII (1)
    HindIII-HF®HphIHpy188IIIHpyAVI-CeuI
    I-SceIKasILpnPIMmeIMnlI
    MscIMspA1IMspJIMwoINcoI-HF® (2)
    NlaIIINlaIVNmeAIIINsiI (1)NsiI-HF®
    Nt.BspQIPciIPI-PspIPI-SceIPpuMI
    PsiIPspOMIPspXIPvuI (1)PvuI-HF®
    PvuII (1)PvuII-HF®SapISbfI-HF®ScaI-HF® (2)
    SfaNISfcISfoISmaISphI (1)
    SphI-HF®SspI-HF®StyD4ISwaITaqαI
    TliITseITspMITspRITth111I
    ZraI
    Diluent Buffer C
    AbaSIAgeI (1) (2)BciVIBsaAIBsrFI
    CviAIICviQIEagI (1)EcoRI (1) (2)EcoRI-HF® (2)
    FspIHpy166IIMboIINheI (1) (2)NheI-HF® (2)
    NotI (1) (2)PstI (1)PstI-HF®RsrIIScrFI
    SfiISpeI (1) (2)SpeI-HF®SspI (1)TfiI
    XcmI
    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.
    HF® is a registered trademark of New England Biolabs, Inc.