Enzymes with Nonpalindromic Sequences
| Enzyme | Recognition Sequence |
|---|---|
| AciI | CCGC(-3/-1) |
| AcuI | CTGAAG(16/14) |
| AlwI | GGATC(4/5) |
| BaeI | (10/15)ACNNNNGTAYC(12/7) |
| BbsI § | GAAGAC(2/6) |
| BbsI-HF® | GAAGAC(2/6) |
| BbvCI | CCTCAGC(-5/-2) |
| BbvI | GCAGC(8/12) |
| BccI | CCATC(4/5) |
| BceAI | ACGGC(12/14) |
| BcgI | (10/12)CGANNNNNNTGC(12/10) |
| BciVI | GTATCC(6/5) |
| BcoDI | GTCTC(1/5) |
| BfuAI | ACCTGC(4/8) |
| BmgBI | CACGTC(-3/-3) |
| BmrI | ACTGGG(5/4) |
| BpmI | CTGGAG(16/14) |
| BpuEI | CTTGAG(16/14) |
| Bpu10I | CCTNAGC(-5/-2) |
| BsaI-HF®v2 | GGTCTC(1/5) |
| BsaXI | (9/12)ACNNNNNCTCC(10/7) |
| BseRI | GAGGAG(10/8) |
| BseYI | CCCAGC(-5/-1) |
| BsgI | GTGCAG(16/14) |
| BsmAI | GTCTC(1/5) |
| BsmBI-v2 | CGTCTC |
| BsmFI | GGGAC(10/14) |
| BsmI | GAATGC(1/-1) |
| BspCNI | CTCAG(9/7) |
| BspMI | ACCTGC(4/8) |
| BspQI | GCTCTTC(1/4) |
| BspQI-HF® § | GCTCTTC(1/4) |
| BsrBI | CCGCTC(-3/-3) |
| BsrDI | GCAATG(2/0) |
| BsrI | ACTGG(1/-1) |
| BssSI-v2 | CACGAG(-5/-1) |
| BtgZI | GCGATG(10/14) |
| BtsCI | GGATG(2/0) |
| BtsIMutI | CAGTG(2/0) |
| BtsI-v2 | GCAGTG(2/0) |
| CspCI | (11/13)CAANNNNNGTGG(12/10) |
| EarI | CTCTTC(1/4) |
| EciI | GGCGGA(11/9) |
| EcoP15I | CAGCAG(25/27) |
| Esp3I | CGTCTC(1/5) |
| FauI | CCCGC(4/6) |
| FokI | GGATG(9/13) |
| HgaI | GACGC(5/10) |
| HphI | GGTGA(8/7) |
| HpyAV | CCTTC(6/5) |
| I-CeuI | TAACTATAACGGTCCTAAGGTAGCGAA(-9/-13) |
| I-SceI | TAGGGATAACAGGGTAAT(-9/-13) |
| MboII | GAAGA(8/7) |
| MlyI | GAGTC(5/5) |
| MmeI | TCCRAC(20/18) |
| MnlI | CCTC(7/6) |
| MspJI | CNNR(9/13) |
| Nb.BbvCI | CCTCAGC(none/-2) |
| Nb.BsmI | GAATGC(none/-1) |
| Nb.BsrDI | GCAATG(none/0) |
| Nb.BssSI | CACGAG(none/-1) |
| Nb.BtsI | GCAGTG(none/0) |
| NmeAIII | GCCGAG(21/19) |
| Nt.AlwI | GGATC(4/none) |
| Nt.BbvCI | CCTCAGC(-5/none) |
| Nt.BsmAI | GTCTC(1/none) |
| Nt.BspQI | GCTCTTC(1/none) |
| Nt.BstNBI | GAGTC(4/none) |
| Nt.CviPII | CCD(-3/none) |
| PaqCI | CACCTGC(4/8) |
| PI-PspI | TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17) |
| PI-SceI | ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19) |
| PleI | GAGTC(4/5) |
| SapI | GCTCTTC(1/4) |
| SfaNI | GCATC(5/9) |
| WarmStart® Nt.BstNBI | GAGTC(4/none) |
§ A HF version of this enzyme is available.
