Enzymes with Nonpalindromic Sequences

All recognition sequences are written 5' to 3' using the single letter code nomenclature. Numbers in parentheses indicate the point of cleavage.

Example, GGTCTC(1/5) indicates cleavage at:
5'...GGTCTCN/...3'
3'...CCAGAGNNNN/...5'

Enzyme Recognition Sequence
AatII GACGT/C
AccI GT/MKAC
Acc65I G/GTACC
AclI AA/CGTT
AfeI AGC/GCT
AflII C/TTAAG
AflIII A/CRYGT
AgeI-HF® A/CCGGT
AhdI GACNNN/NNGTC
AleI-v2 CACNN/NNGTG
AluI AG/CT
AlwNI CAGNNN/CTG
ApaI GGGCC/C
ApaLI G/TGCAC
ApeKI G/CWGC
ApoI-HF R/AATTY
AscI GG/CGCGCC
AseI AT/TAAT
AsiSI GCGAT/CGC
AvaI C/YCGRG
AvaII G/GWCC
AvrII C/CTAGG
BaeGI GKGCM/C
BamHI § G/GATCC
BamHI-HF® G/GATCC
BanI G/GYRCC
BanII GRGCY/C
BclI § T/GATCA
BclI-HF T/GATCA
BfaI C/TAG
BglI GCCNNNN/NGGC
BglII A/GATCT
BlpI GC/TNAGC
BmtI-HF® GCTAG/C
BsaAI YAC/GTR
BsaBI GATNN/NNATC
BsaHI GR/CGYC
BsaJI C/CNNGG
BsaWI W/CCGGW
BsiEI CGRY/CG
BsiHKAI GWGCW/C
BsiWI § C/GTACG
BsiWI-HF® C/GTACG
BslI CCNNNNN/NNGG
BsoBI C/YCGRG
BspDI AT/CGAT
BspEI T/CCGGA
BspHI T/CATGA
Bsp1286I GDGCH/C
BsrFI-v2 R/CCGGY
BsrGI-HF® T/GTACA
BssHII G/CGCGC
BstAPI GCANNNN/NTGC
BstBI TT/CGAA
BstEII-HF® G/GTNACC
BstNI CC/WGG
BstUI CG/CG
BstXI CCANNNNN/NTGG
BstYI R/GATCY
BstZ17I-HF® GTATAC
Bsu36I CC/TNAGG
BtgI C/CRYGG
Cac8I GCN/NGC
ClaI AT/CGAT
CviKI-1 RG/CY
CviQI G/TAC
DdeI C/TNAG
DpnI GA/TC
DpnII /GATC
DraI TTT/AAA
DraIII-HF® CACNNN/GTG
DrdI GACNNNN/NNGTC
EaeI Y/GGCCR
EagI-HF® C/GGCCG
Eco53kI GAG/CTC
EcoNI CCTNN/NNNAGG
EcoO109I RG/GNCCY
EcoRI § G/AATTC
EcoRI-HF® G/AATTC
EcoRV § GAT/ATC
EcoRV-HF® GAT/ATC
FatI /CATG
Fnu4HI GC/NGC
FseI GGCCGG/CC
FspI TGC/GCA
HaeII RGCGC/Y
HaeIII GG/CC
HhaI GCG/C
HincII GTY/RAC
HindIII § A/AGCTT
HindIII-HF® A/AGCTT
HinfI G/ANTC
HinP1I G/CGC
HpaI GTT/AAC
HpaII C/CGG
HpyCH4III ACN/GT
HpyCH4IV A/CGT
HpyCH4V TG/CA
Hpy99I CGWCG/
Hpy188I TCN/GA
Hpy166II GTN/NAC
Hpy188III TC/NNGA
KasI G/GCGCC
KpnI-HF® GGTAC/C
MboI /GATC
MfeI-HF® C/AATTG
MluCI /AATT
MluI-HF® A/CGCGT
MscI TGG/CCA
MseI T/TAA
MslI CAYNN/NNRTG
MspA1I CMG/CKG
MspI C/CGG
MwoI GCNNNNN/NNGC
NaeI GCC/GGC
NarI GG/CGCC
NciI CC/SGG
NcoI § C/CATGG
NcoI-HF® C/CATGG
NdeI CA/TATG
NgoMIV G/CCGGC
NheI-HF® G/CTAGC
NlaIII CATG/
NlaIV GGN/NCC
NotI § GC/GGCCGC
NotI-HF® GC/GGCCGC
NruI-HF® TCG/CGA
NsiI § ATGCA/T
NsiI-HF® ATGCA/T
NspI RCATG/Y
PacI TTAAT/TAA
PaeR7I C/TCGAG
PciI A/CATGT
PflFI GACN/NNGTC
PflMI CCANNNN/NTGG
PluTI GGCGC/C
PmeI GTTT/AAAC
PmlI CAC/GTG
PpuMI RG/GWCCY
PshAI GACNN/NNGTC
PsiI-v2 TTA/TAA
PspGI /CCWGG
PspOMI G/GGCCC
PspXI VC/TCGAGB
PstI § CTGCA/G
PstI-HF® CTGCA/G
PvuI-HF® CGAT/CG
PvuII § CAG/CTG
PvuII-HF® CAG/CTG
RsaI GT/AC
RsrII CG/GWCCG
SacI-HF® GAGCT/C
SacII CCGC/GG
SalI § G/TCGAC
SalI-HF® G/TCGAC
Sau3AI /GATC
Sau96I G/GNCC
SbfI-HF® CCTGCA/GG
ScaI-HF® AGT/ACT
ScrFI CC/NGG
SexAI A/CCWGGT
SfcI C/TRYAG
SfiI GGCCNNNN/NGGCC
SfoI GGC/GCC
SgrAI CR/CCGGYG
SmaI CCC/GGG
SmlI C/TYRAG
SnaBI TAC/GTA
SpeI-HF® A/CTAGT
SphI § GCATG/C
SphI-HF® GCATG/C
SrfI GCCC/GGGC
SspI-HF® AAT/ATT
StuI AGG/CCT
StyD4I /CCNGG
StyI-HF® C/CWWGG
SwaI ATTT/AAAT
TaqI-v2 T/CGA
TfiI G/AWTC
TseI G/CWGC
Tsp45I /GTSAC
TspMI C/CCGGG
TspRI NNCASTGNN/
Tth111I GACN/NNGTC
XbaI T/CTAGA
XcmI CCANNNNN/NNNNTGG
XhoI C/TCGAG
XmaI C/CCGGG
XmnI GAANN/NNTTC
ZraI GAC/GTC
§ A HF version of this enzyme is available.