All restriction endonuclease recognition specificities available from New England Biolabs are listed below. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. For example, CCTC(7/6) and (6/7)GAGG both represent an MnlI (NEB #R0163) site.
All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".
Numbers in parentheses indicate point of cleaveage for non-palindromic enzymes.
For example, GGTCTC(1/5) indicates cleavage at: 5´ ...GGTCTCN/...3´ 3´ ...CCAGAGNNNNN/...5´
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