Monarch^® HMW gDNA Tissue Lysis Buffer

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T3061L     25       Monarch® HMW gDNA Tissue Lysis Buffer T3061-1 25 2 x 31 ml Not Applicable

Companion Products

• Monarch HMW DNA Extraction Kit for Tissue
• Monarch Pestle Set
• Monarch 2 ml Tubes
• Monarch Bead Retainers
• Monarch DNA Capture Beads
• Monarch Protein Separation Solution
• Monarch gDNA Elution Buffer II
• Monarch^® Collection Tubes II
• Proteinase K, Molecular Biology Grade
• Monarch gDNA Wash Buffer

1. Download Quick Protocol Card for Tissue
2. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
3. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
4. Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
5. Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies^® UL Library Prep Workflow

• A faster workflow for the assessment of genomic loci in mice using a novel HMW DNA extraction technology upstream of Cas9 targeted sequencing

1. Can I use the Monarch HMW DNA Extraction Kits for processing plant samples?
2. Can I use the Monarch HMW DNA Extraction Kits for processing insect samples?
3. Can the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
4. Can the Monarch HMW DNA Extraction Kits be used to process marine samples, including marine invertebrates?
5. Can the Monarch HMW DNA Extraction Kits be used to process environmental or fecal samples for metagenomic studies?
6. Can the Monarch HMW DNA Extraction Kits be used to isolate BAC DNA?
7. How do you suggest homogenizing tougher tissues, like ear punches or muscle? Can a sonicator be used for tissue homogenization?
8. Does the kit work with frozen tissue, tissue stored in preservatives (ethanol, DMSO-salt/DESS, or RNAlater^®), FFPE samples, or OCT-preserved tissues?
9. Do you see different fragmentation results when using the pestle versus the rotor-stator homogenizer for tissue homogenization?

• Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T3061L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T3061L_v1_10119975
• T3061L_v1_10152953

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® HMW gDNA Tissue Lysis Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.