High Molecular Weight DNA Extraction
T
he Monarch® HMW DNA Extraction Kits provide a rapid and reliable process for extracting high molecular weight DNA (HMW DNA) from biological samples. NEB offers two approaches for the extraction and purification of gDNA: the Monarch Genomic DNA Purification Kit, which is a silica spin column-based process, and the Monarch HMW DNA Extraction Kits, which utilize a novel glass bead-based process for the purification of extremely high molecular weight DNA.
These kits utilize an optimized process that combines gentle cell lysis with a tunable fragment length generation, followed by precipitation of the extracted DNA onto the surface of large glass beads. Extracted DNA size ranges from 50-250 kb for the standard protocol and into the Mb range for certain sample types when the lowest agitation speeds are used.
Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA, and is ready for use in downstream applications, including long-read sequencing.
Extract HMW DNA from cells and blood reproducibly with Monarch HMW DNA Extraction Kits
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- Which measurement system is best used for assessing the concentration of HMW DNA?
- Are the Monarch HMW DNA Extraction kits compatible with samples stored in Monarch DNA/RNA Protection Reagent, RNAlater, DNA/RNA Shield etc.?
- Can I use the Monarch HMW DNA Extraction Kits for processing plant samples?
- Can I use the Monarch HMW DNA Extraction Kits for processing insect samples?
- Can the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
- Can the Monarch HMW DNA Extraction Kits be used to process environmental or fecal samples for metagenomic studies?
- Can the Monarch HMW DNA Extraction Kits be used to process marine samples, including marine invertebrates?
- Do you have any PacBio Sequencing results from DNA extracted from tissue samples with the Monarch HMW DNA Extraction Kits?
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Blood (NEB #T3050)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Tissue (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Bacteria (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Yeast (NEB #T3060)
- Protocol for High Molecular Weight DNA (HMW DNA) Extraction from Phage
- Protocol for UHMW DNA Cleanup in the Oxford Nanopore Technologies® UL Library Prep Workflow
- Monarch DNA Purification Brochure
- Troubleshooting Guide for Monarch HMW DNA Extraction Kits (NEB #T3050 and #T3060)
- Choosing Input Amounts for the Monarch HMW DNA Extraction Kits
- Considerations and Performance Data for Nanopore Sequencing of High Molecular Weight DNA (HMW DNA)
- Important Considerations for Starting Materials When Using the Monarch HMW DNA Extraction Kit for Cells & Blood (NEB #T3050)
- Measuring, Analyzing & Storing High Molecular Weight DNA (HMW DNA) Samples
Brochures
Troubleshooting Guides
Usage Guidelines
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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