The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic
DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria
and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Protocols are also included to enable
purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA.
Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The
purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has
a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms.
Cultured mammalian cells: up to 5 x 106 cells
Mammalian whole blood: 100 µl
Tissue: up to 25 mg, depending on tissue type
Bacteria: up to 2 x 109
Yeast: up to 5 x 107
Saliva: up to 500 µl
Genomic DNA requiring cleanup
30 µg genomic DNA
Varies depending on sample type
Genomic DNA Size:
Peak size > 50 kb for most sample types; may be lower for saliva and buccal swabs
< 1% (with included RNase A treatment)
≥35 µl, but 100 µl is recommended
A260/280 ≥ 1.8
A260/230 ≥ 2.0
Validated Sample Types:
Guinea Pig Blood
The following reagents are supplied with this product:
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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