
Genomic DNA Extraction & Purification
The ability to extract and purify gDNA from samples of interest is an essential first step in the study of many biological processes. Purification of gDNA often begins with homogenization of the sample followed by lysis of the cells to release the gDNA. RNase is typically employed to remove contaminated RNA from the lysate. The gDNA is then conditioned to selectively bind to a capture column, where it is subsequently washed to remove salts and other contaminants prior to elution.
NEB offers two approaches for the extraction and purification of gDNA: the Monarch Spin gDNA Purification Kit, which is a silica spin column-based process and the Monarch HMW DNA Extraction Kits, which utilize a novel glass bead-based process for purification of extremely high molecular weight DNA. The Monarch Spin gDNA Purification Kit is a universal kit for DNA extraction and purification from a wide variety of cell types, including blood, cells, tissues and tough-to-lyse samples (including bacteria and yeast).
The kit includes lysis buffers for various input materials, Proteinase K for homogenization of some sample types, and RNase A for efficient RNA removal. Genomic DNA (gDNA) is eluted with high yield and purity, and with excellent integrity (high molecular weight), ready for use in downstream applications including qPCR, long range PCR, and NGS library prep (including for long read sequencing platforms).
Choose Monarch for genomic DNA extraction and purification from a wide range of sample types

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- What size gDNA can be purified with the Monarch® Spin gDNA Extraction Kit?
- Can I use the Monarch® Spin gDNA Extraction Kit for gDNA cleanup?
- How can I assess gDNA integrity and purity?
- Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used to isolate gDNA from drosophila and other insects?
- Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used to isolate gDNA from plants?
- Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used to isolate gDNA from plasma, serum and urine?
- What is the minimum elution volume that can be used with the Monarch® Spin gDNA Extraction Kit columns?
- Is Monarch-purified gDNA suitable for long-range PCR?
- Can I purchase Monarch® buffers and columns separately?
- When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
- Can I use Monarch® RNase A for applications/workflows other than gDNA purification?
- Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
- Can the Monarch® Spin gDNA Extraction Kit (NEB #T3010) be used with a vacuum manifold?
- Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
- Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
- Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
- Genomic DNA Extraction from Gram-negative Bacteria (NEB #T3010)
- Genomic DNA Extraction from Gram-positive Bacteria and Archaea (NEB #T3010)
- Genomic DNA Extraction from Saliva (NEB #T3010)
- Genomic DNA Extraction from Yeast (NEB #T3010)
- Genomic DNA Cleanup Protocol
- Quick Protocol for Extraction and Purification of Genomic DNA
- Genomic DNA Extraction from Insects
- Monarch DNA Purification Brochure
- Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)
- Choosing Input Amounts for the Monarch Spin gDNA Extraction Kit
- Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch® Spin gDNA Extraction Kit
- Guidelines for Handling Tissue Samples when using the Monarch Spin gDNA Extraction Kit
Brochures
Troubleshooting Guides
Usage Guidelines
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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