CLIP-Cell™ TMR-Star

CLIP-Cell™ TMR-Star is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro.

  • This is a cell-permeable substrate (BC-TMR) based on tetramethylrhodamine
  • It is suitable for standard rhodamine filter sets
  • It has an excitation maximum at 554 nm and emission maximum at 580 nm

Ordering Information

$name
  • 30 nmol
    $307.00
  • Product Information
    This package includes 30 nmol of CLIP-Cell TMR-Star, sufficient to make 10 ml of a 3 µM CLIP-tag fusion protein labeling solution.

    The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNAalkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond.  Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells. 

    There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins with the CLIP-tag substrate is described in this document.

    Live COS-7 expressing pCLIP-H2B and pSNAP-ADRβ2 were labeled with CLIP-Cell TMR-Star (red) and SNAP-Alexa Fluor® 488 (green) for 60 minutes.

    Excitation (dotted line) and emission spectra of CLIP-Cell TMR-Star coupled to CLIP-tag in buffer at pH 7.5.
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    Structure of CLIP-Cell TMR-Star (MW 642.7 g/mol)
    Product Categories:
    CLIP-tag™ Substrates Products,
    Protein Labeling Products
    Applications:
    CLIP Cell,
    CLIP Surface,
    In vivo Imaging
    ,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
    • Properties and Usage
    • Notes
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