SNAP-Surface® 649

SNAP-Surface 649 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins on cell surfaces and in solution.

  • This is a non-cell-permeable substrate (BG-649) based on the Dyomics dye DY-649P1
  • It is suitable for excitation with a 650 nm diode laser or use with Cy5 filter sets
  • It has an excitation maximum at 655 nm and emission maximum at 676 nm

Ordering Information

$name
  • 50 nmol
    $307.00
  • Product Information
    This package includes 50 nmol of SNAP-Surface 649 substrate, sufficient to make 10 ml of a 5 μM SNAP-tag fusion protein labeling solution.

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine- DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAPtag substrate is described below.

    Excitation (dotted line) and emission spectra of SNAP-Surface 649 coupled to SNAP-tag in buffer at pH 7.5Excitation (dotted line) and emission spectra of SNAP-Surface 649 coupled to SNAP-tag in buffer at pH 7.5
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    Structure of SNAP-Surface 649 (MW 1221.3 g/mol).
    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Surface,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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