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BG-Maleimide

BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides.

  • This building block allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of applications
  • It can be used for the coupling of proteins and solid surfaces such as DNA chips

Ordering Information

$name
  • 2 mg
    $362.00
  • Product Information
    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described in this document.

    Figure 1: Structure of BG-Maleimide (MW 489.5 g/mol) Figure 1: Structure of BG-Maleimide (MW 489.5 g/mol)
    Product Categories:
    Building Blocks Products,
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    Cell Imaging,
    Protein Labeling (SNAP/CLIP)
    • Properties and Usage
    • Notes
    • References
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