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  • BG-GLA-NHS

    Description

    BG-GLA-NHS is an amine-reactive building block for the one-step synthesis of SNAP-tag® substrates from amine-containing precursors including proteins, peptides or oligonucleotides. This building block allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins. 

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described in this document.
    S9151a
    Figure 1. Structure of BG-GLA-NHS (MW 481.5 g/mol)

    Notes

    1. BG-GLA-NHS is supplied in lyophilized form and packaged under argon. The dry SNAP-tag building block substrate should be stored at -20°C.
    2. BG-GLA-NHS is soluble in anhydrous DMF or DMSO. Choose a solvent volume that will be compatible with the final use. Vortex and centrifuge briefly to remove any non-dissolved solids.
    3. NHS esters readily hydrolyze in moist environments. Experiments should be designed to maximize the usage of BG-GLA-NHS. Storage of unused BG-GLA-NHS solubilized in DMF or DMSO should be avoided. If unavoidable, we recommend storing at -20°C for the minimum time period.

    References

    1. Howland, S.W. et al. (2008). J. Immunother. 31, 607-619.

    FAQs

    1. Cellular Imaging and Analysis FAQs

    Protocols

    1. Labeling SNAP-tag Fusion Proteins with BG-substrates (S9151)
    2. Reaction Conditions for Chemical Coupling (S9151)

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Troubleshooting Guides

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Physical Purity (HPLC):
      The purity of the product is determined by HPLC analysis.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.