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BG-GLA-NHS

BG-GLA-NHS is an amine-reactive building block for the one-step synthesis of SNAP-tag® substrates from amine-containing precursors including proteins, peptides or oligonucleotides.

  • This building block allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins

Ordering Information

S9151S_pdp_800X450
  • 2 mg
    $362.00
  • Product Information
    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described in this document.
    9147a_thumb
    Figure 1. Structure of BG-GLA-NHS (MW 481.5 g/mol)
     
    Product Categories:
    Building Blocks Products,
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    Protein Labeling (SNAP/CLIP),
    Cell Imaging
    • Properties and Usage
    • Notes
    • References
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