SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells.
- It is largely membrane impermeable essentially limiting blocking to cell surface-exposed SNAP-tags
- Once it reacts with the SNAP-tag, it irreversibly inactivates it for subsequent labeling steps
- It can be used to generate inactive controls in live cell labeling experiments performed with SNAP-tag fusion proteins
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the instructions supplied with SNAP-tag plasmids. The labeling of SNAP-tag fusion proteins with SNAP-Surface substrates is described in the instructions supplied with SNAP-Surface substrates. The use of SNAP-Surface Block during the labeling of fusion proteins with SNAP-Surface substrates is described below.
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