SNAP-Surface® Block

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells.

  • It is largely membrane impermeable essentially limiting blocking to cell surface-exposed SNAP-tags
  • Once it reacts with the SNAP-tag, it irreversibly inactivates it for subsequent labeling steps
  • It can be used to generate inactive controls in live cell labeling experiments performed with SNAP-tag fusion proteins
 

Ordering Information

S9143S_pdp_800X450
  • 200 nmol
    $121.00
  • Product Information
    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the instructions supplied with SNAP-tag plasmids. The labeling of SNAP-tag fusion proteins with SNAP-Surface substrates is described in the instructions supplied with SNAP-Surface substrates. The use of SNAP-Surface Block during the labeling of fusion proteins with SNAP-Surface substrates is described below.
    Figure 1: Structure of SNAP-Surface Block (MW 313.31 g/mol)
    Figure 1: Structure of SNAP-Surface Block (MW 313.31 g/mol)

    Product Categories:
    Blocking Agents Products,
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Surface,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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