Chitin Resin

Description

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion (1)

IMPACT Citations

Properties and Usage

Storage Temperature

4°C

Binding Capacity

2.0 mg maltose-binding protein/ ml bed volume released from the resin after cleavage of the fusion protein expressed from pMYB5.

Can be Regenerated

yes

Notes

  1. We recommend using gravity flow to run the column. The column should have a wide diameter.
  2. The column may be regenerated 5 times.
  3. Store at 4°C. The used resin should be stored in column buffer. For long term storage add 0.02% sodium azide to the column buffer.

References

  1. Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H. and Xu, M.-Q. (1997). Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene.

Protocols

  1. Isolation of CBD-fusion protein using Chitin Magnetic Beads

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Health Warning

  • DANGER
  • Flammable liquid and vapor.
  • Toxic to aquatic life with long lasting effects.