NEBExpress® Ni-NTA Magnetic Beads

  • An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats.
  • Prepared with agarose based, super-paramagnetic microparticles which provide high binding capacity and fast magnetic response permitting high throughput and scalable purification strategies of His tagged-fusion proteins.
  • NTA coordination exhibits low Nickel ion leaching.
  • Tolerates a wide range of conditions, including the presence of protein denaturants and detergents. Compatible with commercially available detergent based cell lysis reagents.
  • Elution of the target protein may be achieved by protonation, ligand exchange (with imidazole) or extraction of the metal ion by a stronger chelator (e.g. EDTA).
  • Extremely low non-specific binding properties permit immobilized fusion proteins to be used in subsequent experiments to capture or pull down interacting proteins from crude cell lysates
Catalog Number Concentration Size List Price Your Price Quantity
S1423S 10 % 1 ml $196.00
S1423L 10 % 5 ml $926.00
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