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  • Ribonucleoside Vanadyl Complex

    Description

    Ribonucleoside-vanadyl complex is a potent inhibitor of various ribonucleases (1,2,3). This complex is compatible with cell fractionation methods as well as sucrose-gradient centrifugations. The 200 mM stock solution is reconstituted to a green-black clear solution by incubating the sealed vial at 65°C. Once open, the entire sample should be aliquoted into smaller samples and frozen.

    The ribonucleoside-vanadyl complex should be added to all buffers to a final concentration of 10 mM. The buffers should not contain EDTA since one equivalent will totally dissociate the complex.

    We do not recommend the use of the vanadyl complex in cell-free translation systems and with reverse transcriptase (5). The vanadyl complex can be used in the selective degradation of DNA while preserving RNA since pancreatic deoxyribonuclease I is not inhibited (5). Removal of the ribonucleoside-vanadyl complex from the RNA can be accomplished by adding 10 equivalents of EDTA before ethanol precipitation.

    Product Source

    This vanadyl complex is prepared from a modification of procedures by Lienhard using all four ribonucleosides (4).

    Properties and Usage

    Storage Temperature

    -20°C

    Shipping Notes

    • Ships on dry ice

    Notes

    1. Concentration of ribonucleoside-complex at 50% inhibition 2.4 mM.
    2. The toxicological properties of this compound have not been fully investigated
    3. Avoid contact with skin.

    References

    1. Berger, S.L. and Birkenmeier, C.S. (1979). Biochemistry. 18
    2. Gray, J.C. (1974). Arch. Biochem. Biophys.. 163, 343-348.
    3. Egberts, E., Hackett, P.B. and Traub, P. (1971). Hoppe-Zeyler's Z. Physiol Chem.. 358, 475-490.
    4. Leinhard, G.E., Secemski, I.I., Koehler, K.A. and Lindquist, R.N. (1971). Cold Spring Harbor Symp. Quant. Biol.. 36, 45-51.
    5. Berger, S.L., Hitchcock, N.J.M., Zoon, K.C., Birkenmier, C.S., Friedman, R.M. and Chang, E.H. (1980). J. Biol. Chem.. 21, 4602-4608.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Please, make sure this product is stored frozen until just before using or aliquotting.
    This product is very susceptible to oxidation so it is recommended to aliquot the solution as needed and avoid refreezing any leftover solution.