Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.
- 100% activity in CutSmart® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Specificity to epigenetically-relevant DNA modifications (5-mC and 5-hmC)
Reduce Star Activity with High-Fidelity Restriction Enzymes
Standard Protocol for Restriction Enzyme Digests
NEB TV Episode 15
CutSmart Restriction Enzyme Buffer
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
Why is My Restriction Enzyme Not Cutting DNA?
Restriction Enzyme Digest Problem: Too Many DNA Bands
Double Digestion with NEBcloner
Recognition sequence: x is ghmC, hmC, mC or C
AbaSI is a DNA modification-dependent endonuclease and it is an EpiMark® validated product. AbaSI recognizes 5-glucosylhydroxymethylcytosine (5ghmC) in double stranded DNA. It also recognizes 5-hydroxymethylcytocine (5hmC) but at a lower efficiency. It does not recognize DNA with 5-methylcytosin or un-modified cytosine. AbaSI selectively cleaves DNA that contains the modified bases, 5ghmC or 5hmC on one or both strands and introduces a double-stranded DNA break on the 3´ side away from the modified cytosine producing a 2-base or 3-base 3´-overhang. Sites with two 5ghmC on opposite strands are cleaved most efficiently; sites with one 5ghmC and another C or 5mC are cleaved less efficiently.
Product SourceAn E. coli strain that carries the synthetic AbaSI gene from Acinetobacter baumannii SDF species.
The following reagents are supplied with this product:
Store at (°C) Concentration CutSmart® Buffer 10 X
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- Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated),
- Restriction Endonucleases: A
- Restriction Enzyme Digestion
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