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This rabbit polycolonal antibody was obtained from the immunization with purified recombinant SNAP-tag® protein and affinity purified using SNAP-BG resin.
Western blot analysis of SNAP-tag fusion proteins expressed in mammalian cells
CHO-K1 cells were transfected with plasmids expressing histone protein H2B or adenoviral protein E3-14.7K fused to SNAP-tag. The cell lysates, each prepared from 5x103 cells, were electrophoresed on a 10–20% Tris-glycine polyacrylamide gel under denaturing conditions. Western blot analysis was performed with anti-SNAP antibody in 1:1000 dilution and chemiluminescent detection. Control: untransfected cell lysate.
This product is related to the following categories:
Storage: This antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, and 50% glycerol. Store undiluted at -20°C for up to two years. Avoid repeated elevation in temperature.
The optimal dilution for Western blotting may have to be experimentally determined to account for protein expression and assay conditions. The recommended starting dilution is 1:1000.
The sensitivity of detection is 10 ng of SNAP-tag per load in Western blotting.
This antibody has not been validated for other applications.
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The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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