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  • Trypsin-digested BSA MS Standard (CAM-modified)

    Description

    A complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM modified). This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap).

    MALDI Analysis of BSA Digest:
    The BSA digest solution was diluted to 1 pmol/μl and mixed at a 1:1 ratio with α-cyano-4-hydroxycinnamicaci (10 mg/ml in 50:50 acetonitrile:water with 0.1% trifluoroacetic acid). One μl of the digest/matrix solution were then spotted directly onto a MALDI target plate. The sample was then analyzed on a MALDI micro MX Mass Spectrometer (Waters Corporation) with a laser energy of 150.
    Online Analysis of BSA Digest:
    The BSA digest solution was diluted to 100 fmol/μl with 0.1% formic acid. One μl (100 fmol) of the digest solution was injected via an Easy-nanoLC II (Proxeon) onto a self-packed reverse phase C18 nano column (Phenomenex packing material, 5 cm, 150 μm) and loaded onto a self-packed C18 analytical column with an integrated tip (New Objective Picofrit, 15 cm, 100 μm, Phenomenex packing material). Peptides were separated using a 45 min 5-70%B gradient (A = 0.1% formic acid, B = CH3CN, 0.1% formic acid) at a flow rate of 400 nl/min. Eluting peptides were analyzed online by a Q-Exactive mass spectrometer (Thermo Scientific) with an electrospray ion source. MS/MS spectra were acquired by HCD using an acquisition range of 400 to 1600 m/z.

    Product Source

    BSA (GENBANK P02769) was digested using Modified Trypsin (TPCK-treated)

    Properties and Usage

    Storage Temperature

    -20°C

    Heat Inactivation

    No

    Notes

    1. Suggested volume to resuspend: 500 µl. Avoid repeated freeze/thaw cyles once in solution.
    2. Supplied in lyophilized form.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Functional Test (MS Standards):
      The peptide mixture is mixed with an acid matrix solution, air-dried and analysed by MALDI-TOF MS to confirm the observed spectra match the theoretical spectra of the peptide mix.
    • Protein Identification (MS/MS):
      The peptide mixture is subjected to nano-reverse-phase liquid chromatography.  Protein identity is confirmed by MS/MS data as analyzed by the Mascot search engine.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.