The new NEBNext® FFPE DNA Repair V2 Module improves FFPE DNA repair through an optimized combination of enzymes, designed to be used upstream of NGS library prep for the repair of several of the most common types of damage caused by FFPE treatment. The latest version, “V2”, offers higher repair efficiency and a more streamlined workflow than the original NEBNext FFPE DNA Repair Mix.
Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair v2 Module
|FFPE DAMAGE TYPE||REPAIRED BY THE FFPE DNA REPAIR v2 MODULE|
|Deamination of cytosine to uracil||Yes|
|Nicks and gaps||Yes|
|Blocked 3´ ends||Yes|
FFPE DNA Repair
- What is the difference between the NEBNext FFPE DNA Repair v2 Module (NEB #E7360) and the NEBNext FFPE DNA Repair Mix (NEB #M6630)?
- When working with fragmented DNA, will the ends be ligated together by the FFPE DNA Repair v2 Module?
- Can the FFPE DNA Repair v2 Mix repair damage in both single- and double-stranded DNA? Or, does it require double stranded DNA as a template?
- Do I always need to use the FFPE DNA Repair Mix when I make libraries with FFPE DNA?
- High Quality Automation Libraries Prepared from a Broad Range of DNA Input Amounts (2017)
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