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  • ΦX174 DNA-Hae III Digest

    Description

    The HaeIII digest of ΦX174 yields 11 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1).

    Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



    N3026_thumb

    φX174 DNA-HaeIII Digest visualized by ethidium bromide staining. 1.7% agarose gel.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Gel Loading Dye, Purple (6X), no SDS256X

    Properties and Usage

    Bases

    FragmentMassbp
    12511,353
    22001,078
    3162872
    4112603
    558310
    6a52281
    6b50271
    743234
    836194
    922118
    101372

    Effective Size Range

    72bp to 1,353bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.
    2. 1X Gel Loading Dye, Purple, no SDS: 
      2.5% Ficoll®-400
      10mM EDTA
      3.3mM Tris-HCl (pH 8.0@25°C)
      0.02% Dye 1
      0.001% Dye 2

    References

    1. Fuchs, C. et al. (1978). Gene. 4, 1-23.
    2. Forster, A.C. et al. (1985). Nucl. Acids Res.. 13, 745-761.

    FAQs

    1. Why is the separation of the lower bands incomplete?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use Midori Green with the DNA Ladders from NEB?
    5. Can I use SYBR® with the DNA Ladders from NEB?

    Protocols

    1. Suggested protocol for loading a DNA Ladder/marker

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Interactive Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.