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  • dATP Solution

    Description

    Contains 0.25 ml of 100 mM ultrapure dATP.

    Supplied in:
    Ultrapure water as a sodium salt at pH 7.4.

    Concentration:
    The dATP Solution is supplied at a concentration of 100 mM (±5 mM) as determined by A260 absorbance.

    Diluent Compatibility:
    Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE (10 mM Tris-HCl, 1 mM EDTA (pH 7.5).

    Properties and Usage

    Storage Temperature

    -20°C

    Notes

    1. For use with any DNA Polymerases from NEB.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Assurance Statement

    • The purity of dATP is ≥ 99% as determined by HPLC analysis.

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • PCR Amplification (dNTPs):
      The dNTPs are tested in a polymerase chain reaction (PCR) using Taq DNA Polymerase, a control template and specific primers, resulting in the expected product.
    • Phosphatase Activity (PNPP):
      The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most phosphatases. After incubation the percent degradation is determined by spectrophotometric analysis of released p-nitrophenol at 405 nm.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.