Cytosine modification at carbon 5 (C5) represents an important epigenetic modification. It is also believed to be the starting substrate for the Ten-Eleven Translocation (TET) family of enzymes and their associated oxidation pathways.
- 5-methyl-dCTP (2'-deoxy-5-methylcytidine 5'-triphosphate) offers the ability to enzymatically make defined, fully methylated, cytosine-substituted DNA, which can be used for a variety of biochemical and cellular applications
- 5-methyl-dCTP is supplied as a 10 mM solution at pH 7. Nucleotide concentration is determined by measurements of absorbance at 260 nm.
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