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  • 5-methyl-dCTP

    Description

    5-methyl-dCTP (2'-deoxy-5-methylcytidine 5'-triphosphate) is supplied as a 10 mM solution at pH 7. Nucleotide concentration is determined by measurements of absorbance at 260 nm.

    Properties and Usage

    Storage Temperature

    -20°C

    Supplied As

    Milli-Q™ water as an ammonium salt

    Molecular Weight

    Theoretical: 481.1 daltons

    Quality Control

    Quality Assurance Statement

    • 5-methyl-dCTP is certified free of nucleases and phosphatases.
    • The purity of dATP is ≥ 99% as determined by HPLC analysis.

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Functional Test (Methylated dNTP):
      The methylated dNTP and the appropriate non-methylated dNTP are tested in a polymerase chain reaction (PCR).  The fragments from each reaction are digested with 5mC methyl sensitive enzymes and electrophoresed on an agarose gel. The methylated dNTP product falied to cut by these enzymes, whereas the dCTP product was digested by both enzymes.

    Notes

    1. Supplied In:
      Milli-Q™ water as an ammonium salt at (pH 7.0)
    2. Formula:
      C10H15N3O13P3 (free acid)
    3. Diluent Compatibility:
      Can be diluted using sterile distilled water, preferably Milli-Q™ water, or can be diluted using sterile TE (10 mM Tris-HCl, 1 mM EDTA (pH 7.5)).

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.