NEBNext^® FFPE DNA Repair Mix

1. Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (E7370)
2. Protocol for use with NEBNext FFPE DNA Repair Mix (M6630) and NEBNext Ultra II DNA library Prep Kit for Illumina (E7645)
3. Protocol for use with NEBNext FFPE DNA Repair Mix (M6630) and NEBNext DNA Library Prep Master Mix Set for Illumina (E6040)
4. NEBNext FFPE DNA Repair Mix (M6630) - Protocol for use with Other User-supplied Library Construction Reagents

1. Will the FFPE DNA Repair Mix ligate my DNA fragments?
2. Will treating my DNA with the FFPE DNA Repair Mix hurt my downsteam reaction?
3. Will the FFPE DNA Repair Mix blunt the ends of the DNA?
4. Does the FFPE DNA Repair Mix insert random nucleotides into the sequence that it repairs?
5. Does the FFPE DNA Repair Mix remove covalent modifications from DNA bases, such as biotin or digoxigenin?
6. Does the FFPE DNA Repair Mix repair DNA-protein crosslinks?
7. Does the FFPE DNA Repair Mix fix blocked 3’ ends?
8. Can the FFPE DNA Repair Mix repair damage in both single and double stranded DNA? Or, does it require double stranded DNA as a template?
9. If I had a DNA template with mutation sites (ie. 8-oxoguanine or deaminated cytosines) that are directly adjacent to each other on opposite strands would treatment with FFPE DNA Repair Mix cause a double strand nick/break?
10. What gap lengths can be repaired with the FFPE DNA Repair Mix?
11. Can the FFPE DNA Repair Mix repair bisulfite treated DNA?
12. When working with fragmented DNA, will the ends be ligated together by the FFPE DNA Repair Mix Reaction Mix?
13. How are abasic sites repaired by the FFPE DNA Repair Mix Reaction Mix?
14. Can I use the FFPE DNA Repair Mix to repair other types of low quality DNA other than FFPE DNA?

Product Citation Tool

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M6630S_L_v1
• M6630S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M6630S_v0_10012241
• M6630G_v0_10012657
• M6630L_v0_10014797
• M6630S_v0_10014811
• M6630L_v0_10015806
• M6630S_v0_10008112
• M6630L_v0_10019187
• M6630S_v0_10012658
• M6630G_v0_10020394
• M6630G_v0_10020954
• M6630S__10008112
• M6630L_v0_10012663
• M6630L_v0_10027640
• M6630S_v0_10027630
• M6630L_v0_10030003
• M6630S_v0_10030634
• M6630L_v0_10033955
• M6630S_v0_10033249
• M6630G_v0_10034227
• M6630L_v0_10039106
• M6630S_v0_10039236
• M6630L_v0_10043884
• M6630G_v0_10036812
• M6630S_v0_10036815
• M6630L_v1_10036818
• M6630G_v0_10050177
• M6630S_v2_10050171
• M6630G_v0_10051505
• M6630L_v2_10051560
• M6630S_v2_10051510
• M6630L_v2_10055212
• M6630L_v2_10056183
• M6630G_v0_10057047
• M6630L_v2_10050874

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext^® FFPE DNA Repair Mix
• NEBNext FFPE DNA Repair Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.