NEBNext® FFPE DNA Repair Mix
The NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples.
Biomek Automated NEBNext Ultra Directional RNA Library Prep Kit for Illumina
The NEBNext FFPE DNA Repair Mix contains enzymes and buffers that are optimized to repair FFPE DNA in next generation sequencing workflow. The mix can be used upstream of any next generation sequencing library construction method, for any sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair Mix
FFPE DAMAGE TYPE REPAIRED BY THE FFPE DNA ENZYME REPAIR MIX? Deamination of cytosine to uracil Yes Nicks and gaps Yes Oxidized bases Yes Blocked 3´ ends Yes DNA fragmentation No DNA-protein crosslinks No
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext FFPE DNA Repair Buffer -20 10 X
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