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Histone H3.3, an H3 variant that is found in all eukaryotes from yeast to human, is replication and cell cycle phase-independent and is the most common H3 in non-dividing cells. It has been shown to be enriched in covalent modifications associated with gene activation (4,5).
Highly pure, recombinant protein
Identity confirmed using Edman Degradation to sequence intact protein; mass confirmed by ESI-TOF MS
Tested for exonuclease, endonuclease and protease contamination
Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer (1,2). It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.
1 mg/ml (66 μM) is calculated using the molar extinction coefficient for Histone H3.3 (3960) and its absorbance at 280 nm (6,7). 1.0 A280 units = 3.8 mg/ml.
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