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  • Histone H10 Human, Recombinant

    Description

    Histone H1 acts on the linker region of polynucleosome DNA to condense the chromatin into structures of ~30 nm (1). It is not necessary for octamer or nucleosome core particle formation. Eight different Histone H1 proteins have been identified in the human genome (2). Histone H10 is a non replication-dependent histone that is highly expressed in cells that have terminally differentiated (3).

    Protein Sequence:
    TENST SAPAA KPKRA KASKK STDHP KYSDM IVAAI QAEKN RAGSS RQSIQ KYIKS HYKVG ENADS QIKLS IKRLV TTGVL KQTKG VGASG SFRLA KSDEP KKSVA FKKTK KEIKK VATPK KASKP KKAAS KAPTK KPKAT PVKKA KKKLA ATPKK AKKPK TVKAK PVKAS KPKKA KPVKP KAKSS AKRAG KKK (Genbank accession number: P07305)

    ESI-TOF Analysis of Histone H10 Human, Recombinant.

    SDS-PAGE analysis of Histone H10 Human, Recombinant.
    Lane 1 and 7: NEB Protein Ladder (NEB #P7703), Lanes 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H10 Human, Recombinant.

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM sodium phosphate
    300 mM NaCl
    1 mM EDTA
    pH 7.0 @ 25°C

    Notes

    1. 1 mg/ml, 48 µM is calculated using the molar extinction coefficient for Histone H1 (3840) and its absorbance at 280 nm (4,5).  1.0 A280 units = 5.4 mg/ml.
    2. There is a small percentage of Histone H10 with a mass of 20863.27 which is a +131 Da difference from the major species. This correlates to Histone H10 with an intact N-terminal methionine (6).

    References

    1. van Holde, K.E. (1989). Chromatin. 1
    2. Marzluff, W.F., et al. (2002). Genomics. 80, 487-497.
    3. Pehrson, J.R. and Cole, R.D. (1982). Biochem. 21, 456-460.
    4. Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. . 182, 319-326.
    5. Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
    6. Qing, X. et al. (2010). Biochemistry. 49, 5588-5599.

    FAQs

    1. Do the histones need to be reconstituted?
    2. What are the recommended histone storage conditions?
    3. Are the histones fusion proteins or tagged proteins?
    4. Can the histones be used as substrates for protein modification enzymes? Which ones?

    Tech Tips

    After thawing on ice, mix well by pipetting the solution up and down. Do not centrifuge.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Exonuclease Activity (Radioactivity Release):
      The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
    • Molecular Weight Determination (Mass Spectrometry) :
      The molecular weight of the product is determined using mass spectrometry.
    • Protease Activity (SDS-PAGE):
      The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable degradation of the proteins as determined by SDS-PAGE.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.